Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups

Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English, French

Published date

11-07-2012

Publisher

International Organization for Standardization

ISO/TR 13136:2012 describes the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.

In any case, when one or both of the stx genes is/are detected, the isolation of the strain is attempted.

The isolation of STEC from samples positive for the presence of the genes specifying the serogroups in the scope of this method can be facilitated by using serogroup-specific enrichment techniques (e.g. immunomagnetic separation, IMS).

The protocol uses real-time PCR as the reference technology for detection of the virulence and serogroup-associated genes.

ISO/TR 13136:2012 is applicable to: 1) products intended for human consumption and the feeding of animals; 2) environmental samples in the area of food production and food handling; 3) environmental samples in the area of primary production.

DocumentType	Technical Specification
Pages	22
PublisherName	International Organization for Standardization
Status	Current

Standards	Relationship
XP CEN ISO/TS 13136 : 2012 XP	Identical
NEN NPR CEN ISO/TS 13136 : 2012	Identical
S.R. CEN ISO/TS 13136:2012	Identical
UNI CEN ISO/TS 13136 : 2013	Identical
IS 16987 : 2018	Identical
PD CEN ISO/TS 13136:2012	Identical
CEN ISO/TS 13136:2012	Identical
DIN CEN ISO/TS 13136;DIN SPEC 10794:2013-04	Identical
DIN CEN ISO/TS 13136;DIN SPEC 10794:2013-04	Identical

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 16140-2:2016	Microbiology of the food chain Method validation Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method
ISO/TS 19036:2006	Microbiology of food and animal feeding stuffs Guidelines for the estimation of measurement uncertainty for quantitative determinations
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 7550:1985	Laboratory glassware — Disposable micropipettes
ISO 20837:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 16140:2003	Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods
ISO 16654:2001	Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157