S.R. CEN ISO/TS 13136:2012
Current
The latest, up-to-date edition.
MICROBIOLOGY OF FOOD AND ANIMAL FEED - REAL-TIME POLYMERASE CHAIN REACTION (PCR)-BASED METHOD FOR THE DETECTION OF FOOD-BORNE PATHOGENS - HORIZONTAL METHOD FOR THE DETECTION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) AND THE DETERMINATION OF O157, O111, O26, O103 AND O145 SEROGROUPS (ISO/TS 13136:2012)
Hardcopy , PDF
English
01-01-2012
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents, culture media and reagents
6 Equipment
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Performance data
Annex A (normative) - Flow diagram of the screening
procedure
Annex B (normative) - Flow diagram of the isolation
and confirmation procedure
Annex C (informative) - Identification of Shiga
toxin-producing Escherichia coli (STEC)
by multiplex PCR amplification of virulence
genes and detection of PCR products by
agarose gel electrophoresis
Annex D (informative) - Internal amplification control
Annex E (informative) - Primers and probes for the
PCR assays
Annex F (normative) - Isolation of STEC strains
Bibliography
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