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ASTM D 6974 : 2003

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels-Filtration and Culture Procedures
Available format(s)

Hardcopy , PDF

Superseded date

11-11-2014

Language(s)

English

Published date

11-01-2003

1.1 This practice covers a membrane filter (MF) procedure for the detection and enumeration of Heterotrophic bacteria (HPC) and fungi in liquid fuels with kinematic viscosities 24 mm2 s-1 at ambient temperature.

1.2 This quantitative practice is drawn largely from IP Method 385 and Test Method D 5259.

1.3 This test may be performed either in the field or in the laboratory.

1.4 The ability of individual microbes to form colonies on specific growth media depends on the taxonomy and physiological state of the microbes to be enumerated, the chemistry of the growth medium, and incubation conditions. Consequently, test results should not be interpreted as absolute values. Rather they should be used as part of a diagnostic or condition monitoring effort that includes other test parameters, in accordance with Guide D 6469.

1.5 This practice offers alternative options for delivering fuel sample microbes to the filter membrane, volumes or dilutions filtered, growth media used to cultivate fuel-borne microbes, and incubation temperatures. This flexibility is offered to facilitate diagnostic efforts. When this practice is used as part of a condition monitoring program, a single procedure should be used consistently.

1.6 The values stated in SI units are to be regarded as the standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
D 02
DocumentType
Standard Practice
Pages
5
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

ASTM D 7464 : 2014 Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ASTM E 1259 : 2018 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
ASTM D 7978 : 2014 : R2019 Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
ASTM D 7847 : 2017 Standard Guide for Interlaboratory Studies for Microbiological Test Methods
ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 5465 : 2016 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM E 3152 : 2018 Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
ASTM E 2800 : 2011 : R2017 Standard Practice for Characterization of <emph type="ital">Bacillus</emph> Spore Suspensions for Reference Materials

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US$75.00
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