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ASTM D 7978 : 2014

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Test Method for Determination of the Viable Aerobic Microbial Content of Fuels and Associated Water—Thixotropic Gel Culture Method
Available format(s)

Hardcopy , PDF

Superseded date

09-02-2020

Language(s)

English

Published date

12-15-2014

Publisher

American Society for Testing and Materials

This test method describes a procedure that can be used in the field or in a laboratory to quantify culturable, viable aerobic microorganisms present as contaminants in liquid fuels, including those blended with synthesized hydrocarbons or biofuels, with kinematic viscosities (at 40 °C) of ≤24 mm2 s-1 and heavy and residual fuels with kinematic viscosities (at 40 °C) of ≤700 mm2 s-1 and in fuel-associated water.

1.1This test method describes a procedure that can be used in the field or in a laboratory to quantify culturable, viable aerobic microorganisms present as contaminants in liquid fuels, including those blended with synthesized hydrocarbons or biofuels, with kinematic viscosities (at 40 °C) of ≤24 mm2 s-1 and heavy and residual fuels with kinematic viscosities (at 40 °C) of ≤700 mm2 s-1 and in fuel-associated water.

1.1.1This test method has been validated by an ILS for a range of middle distillate fuels meeting Specifications D975, D1655, ISO 8217 DMA, and NATO F-76.2

1.2This test method quantitatively assesses culturable, viable aerobic microbial content present in the form of bacteria, fungi, and fungal spores. Results are expressed as the total number of microbial colony forming units (CFU)/L of fuel or total number of CFU/mL of associated water. The number of CFU should not be interpreted as absolute values but should be used as part of a diagnostic or condition monitoring effort; for example, these values can be used to assess contamination as absent, light, moderate, or heavy.

Note 1:This test method is technically equivalent to IP 613, although the two methods are not currently jointed.

1.3The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.4This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
D 02
DocumentType
Test Method
Pages
9
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

ASTM D 7687 : 2017 : REDLINE Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
SAE J 313 : 2017 DIESEL FUELS
ASTM D 7847 : 2017 : REDLINE Standard Guide for Interlaboratory Studies for Microbiological Test Methods
ASTM D 6224 : 2016 Standard Practice for In-Service Monitoring of Lubricating Oil for Auxiliary Power Plant Equipment
ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
ASTM D 7847 : 2017 Standard Guide for Interlaboratory Studies for Microbiological Test Methods
ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM E 1259 : 2018 Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C
ASTM D 6469 : 2017 : REDLINE Standard Guide for Microbial Contamination in Fuels and Fuel Systems
ASTM D 8070 : 2016 Standard Test Method for Screening of Fuels and Fuel Associated Aqueous Specimens for Microbial Contamination by Lateral Flow Immunoassay
ASTM D 6224 : 2016 : REDLINE Standard Practice for In-Service Monitoring of Lubricating Oil for Auxiliary Power Plant Equipment

ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water
ASTM D 7464 : 2014 : REDLINE Standard Practice for Manual Sampling of Liquid Fuels, Associated Materials and Fuel System Components for Microbiological Testing
ISO 8217:2017 Petroleum products Fuels (class F) Specifications of marine fuels
ASTM E 2756 : 2010 Standard Terminology Relating to Antimicrobial and Antiviral Agents
ASTM D 7847 : 2017 : REDLINE Standard Guide for Interlaboratory Studies for Microbiological Test Methods
ASTM D 6974 : 2016 : REDLINE Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
ASTM D 6469 : 2017 : REDLINE Standard Guide for Microbial Contamination in Fuels and Fuel Systems

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