• ASTM E 1326 : 2015 : REV A

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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    Standard Guide for Evaluating Non-culture Microbiological Tests

    Available format(s):  Hardcopy, PDF

    Superseded date:  12-15-2020

    Language(s):  English

    Published date:  10-30-2015

    Publisher:  American Society for Testing and Materials

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    1.1The purpose of this guide is to assist users and producers of non-culture microbiological tests in determining the applicability of the test for processing different types of samples and evaluating the accuracy of the results. Culture test procedures such as the Heterotrophic (Standard) Plate Count, the Most Probable Number (MPN) method and the Spread Plate Count are widely cited and accepted for the enumeration of microorganisms. However, these methods have their limitations, such as performance time. Moreover, any given culture test method typically recovers only a portion of the total viable microbes present in a sample. It is these limitations that have recently led to the marketing of a variety of non-culture procedures, test kits and instruments.

    1.2Culture test methods estimate microbial population densities based on the ability of mircoorganisms in a sample to proliferate in or on a specified growth medium, under specified growth conditions. Non-culture test methods attempt to provide the same or complimentary information through the measurement of a different parameter. This guide is designed to assist investigators in assessing the accuracy and precision of non-culture methods intended for the determination of microbial population densities or activities.

    1.3It is recognized that the Heterotrophic Plate Count (HPC) does not recover all microorganisms present in a product or a system (1, 2).2 When this problem occurs during the characterization of a microbiological population, alternative standard enumeration procedures may be necessary, as in the case of sulfate-reducing bacteria. At other times, chemical methods that measure the rates of appearance of metabolic derivatives, the utilization of contaminated product components or genetic profile of the microbial population might be indicated. In evaluating non-culture tests, it is possible that the use of these alternative standard procedures might be the only means available for establishing correlation. In such cases, this guide can serve as a reference for those considerations.

    1.4Because there are so many types of tests that could be considered non-culture based, it is impossible to recommend a specific test protocol with statistical analyses for evaluating the tests. Instead, this guide should assist in determining what types of tests should be considered to verify the utility and identify the limitations of the nonconventional test.

    1.5The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    General Product Information - (Show below) - (Hide below)

    Committee E 35
    Document Type Guide
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM D 6469 : 2017 Standard Guide for Microbial Contamination in Fuels and Fuel Systems
    ASTM E 2275 : 2014 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
    ASTM E 979 : 2009 : R2015 Standard Practice for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
    ASTM E 2169 : 2017 Standard Practice for Selecting Antimicrobial Pesticides for Use in Water-Miscible Metalworking Fluids
    ASTM D 7847 : 2017 Standard Guide for Interlaboratory Studies for Microbiological Test Methods
    ASTM E 3152 : 2018 Standard Guide for Standard Test Methods and Practices Available for Determining Antifungal Activity on Natural or Synthetic Substrates Treated with Antimicrobial Agents
    ASTM D 6974 : 2016 Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures
    ASTM E 2694 : 2016 Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM D 5465 : 1993 : R2012 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
    ASTM D 4012 : 1981 : R2009 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 1129 : 2003 Standard Terminology Relating to Water
    ASTM D 1129 : 2013 Standard Terminology Relating to Water
    ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
    ASTM D 4012 : 1981 : R2002 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 5465 : 2016 : R2020 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
    ASTM D 1129 : 2006 Standard Terminology Relating to Water
    ASTM D 5465 : 1993 : R2004 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
    ASTM E 2756 : 2019 Standard Terminology Relating to Antimicrobial and Antiviral Agents
    ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
    ASTM D 5245 : 2019 Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
    ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
    ASTM E 1601 : 2019 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 5245 : 1992 : R2005 Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
    ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2002 Standard Terminology Relating to Water
    ASTM E 1601 : 1998 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 5245 : 1992 : R1998 Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
    ASTM E 2756 : 2010 Standard Terminology Relating to Antimicrobial and Antiviral Agents
    ASTM E 1601 : 2010 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 5245 : 1992 : R2012 Standard Practice for Cleaning Laboratory Glassware, Plasticware, and Equipment Used in Microbiological Analyses
    ASTM E 1601 : 2012 Standard Practice for Conducting an Interlaboratory Study to Evaluate the Performance of an Analytical Method
    ASTM D 1129 : 2004 Standard Terminology Relating to Water
    ASTM D 4012 : 1981 : R1997 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 5465 : 2016 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
    ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
    ASTM E 2756 : 2018 Standard Terminology Relating to Antimicrobial and Antiviral Agents
    ASTM D 5465 : 1993 : R1998 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
    ASTM D 1129 : 2010 Standard Terminology Relating to Water
    ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
    ASTM D 1129 : 2001 Standard Terminology Relating to Water
    ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
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