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ASTM E 2563 : 2023

Current

Current

The latest, up-to-date edition.

Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

10-06-2023

1.1This practice covers the detection and enumeration of viable and culturable rapidly growing Mycobacteria (RGM), or non-tuberculosis Mycobacteria (NTM) in aqueous metalworking fluids (MWF) in the presence of high non-mycobacterial background population using standard microbiological culture methods.

1.2The detection limit is one colony forming unit (CFU)/mL metalworking fluid.

1.3This practice involves culture of organisms classified as Level 2 pathogens, and should be undertaken by a trained microbiologist in an appropriately equipped facility. The microbiologist should also be capable of distinguishing the diverse colonies of Mycobacteria from other microorganism colonies on a Petri dish and capable of confirming Mycobacteria by acid-fast staining method.

1.4This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.5This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Committee
E 34
DocumentType
Standard Practice
Pages
3
PublisherName
American Society for Testing and Materials
Status
Current
Supersedes

ASTM E 2275 : 2019 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
ASTM E 2523 : 2013 : R2018 Standard Terminology for Metalworking Fluids and Operations
ASTM D 5465 : 2016 : R2020 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM E 2148 : 2021 Standard Guide for Using Documents Related to Metalworking or Metal Removal Fluid Health and Safety

ASTM E 2523 : 2013 : R2018 Standard Terminology for Metalworking Fluids and Operations
ASTM E 2523 : 2023 Standard Terminology for Metalworking Fluids and Operations

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