• There are no items in your cart

ASTM E 2647 : 2020

Current
Current

The latest, up-to-date edition.

Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown Using Drip Flow Biofilm Reactor with Low Shear and Continuous Flow
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

05-01-2020

1.1This test method specifies the operational parameters required to grow a repeatable2 Pseudomonas aeruginosa biofilm close to the air/liquid interface in a reactor with a continuous flow of nutrients under low fluid shear conditions. The resulting biofilm is representative of generalized situations where biofilm exists at the air/liquid interface under low fluid shear rather than representative of one particular environment.

1.2This test method uses the drip flow biofilm reactor. The drip flow biofilm reactor (DFR) is a plug flow reactor with laminar flow resulting in low fluid shear. The reactor is versatile and may also be used for growing and/or characterizing biofilms of different species, although this will require changing the operational parameters to optimize the method based upon the growth requirements of the new organism.

1.3This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log colony forming units per surface area.

1.4Basic microbiology training is required to perform this test method.

1.5The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.7This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Committee
E 35
DocumentType
Test Method
Pages
6
PublisherName
American Society for Testing and Materials
Status
Current
Supersedes

ASTM D 5465 : 2016 : R2020 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM D 5465 : 2016 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

View more information
US$63.00
Excluding Tax where applicable

Access your standards online with a subscription

Features

  • Simple online access to standards, technical information and regulations.

  • Critical updates of standards and customisable alerts and notifications.

  • Multi-user online standards collection: secure, flexible and cost effective.