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BS EN 13784:2002

BS EN 13784:2002

Current

Current

The latest, up-to-date edition.

Foodstuffs. DNA comet assay for the detection of irradiatied foodstuffs. Screening method

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-31-2002

Publisher

British Standards Institution

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Evaluation
8 Limitations
9 Validation
10 Test report
Annex A (informative) Figures
Bibliography

Defines a screening method for foods that contain DNA. It is based on micro-gel electrophoresis of single cells or nuclei to detect DNA fragmentation presumptive to irradiation treatment [1] to [8].

This European Standard specifies a screening method for foods which contain DNA. It is based on micro-gel electrophoresis of single cells or nuclei to detect DNA fragmentation presumptive to irradiation treatment [1] to [8]. The DNA Comet Assay is not radiation specific, therefore, it is recommended to confirm positive results using a standardized method to specifically prove an irradiation treatment of the respective food, e.g. EN 1784, EN 1785, EN 1786, EN 1787, EN 1788, EN 13708, and prEN 13751. Interlaboratory studies have been successfully carried out with a number of food products, both of animal and plant origin such as various meats [9] to [11], seeds, dried fruits and spices [6], [12]. Other studies [13] to [32] demonstrate that the method is applicable to a large variety of foodstuffs, but also that limitations exist (see clause 8).

Committee
AW/275
DevelopmentNote
Supersedes 99/715458 DC. (02/2002)
DocumentType
Standard
Pages
20
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
NBN EN 13784 : 2002 Identical
UNE-EN 13784:2002 Identical
EN 13784:2001 Identical
NS EN 13784 : 1ED 2002 Identical
NF EN 13784 : 2002 Identical
NEN EN 13784 : 2001 Identical
UNI EN 13784 : 2002 Identical
I.S. EN 13784:2002 Identical
SN EN 13784 : 2001 Identical
DIN EN 13784:2002-04 Identical

PREN 13751 : DRAFT 2008 FOODSTUFFS - DETECTION OF IRRADIATED FOOD USING PHOTOSTIMULATED LUMINESCENCE
EN 1787:2000 (edition 2) Foodstuffs - Detection of irradiated food containing cellulose by ESR spectroscopy
EN 1788:2001 Foodstuffs - Thermoluminescence detection of irradiated food from which silicate minerals can be isolated
EN 1785:2003 Foodstuffs - Detection of irradiated food containing fat - Gas chromatographic/mass spectrometric analysis of 2-alkylcyclobutanones
EN 13708:2001 FOODSTUFFS - DETECTION OF IRRADIATED FOOD CONTAINING CRYSTALLINE SUGAR BY ESR SPECTROSCOPY
EN 1784:2003 Foodstuffs - Detection of irradiated food containing fat - Gas chromatographic analysis of hydrocarbons
EN 1786:1996 Foodstuffs - Detection of irradiated food containing bone - Method by ESR spectroscopy
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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PDF - English
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