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BS EN 17049:2018

BS EN 17049:2018

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Current

The latest, up-to-date edition.

Animal feeding stuffs. Methods of sampling and analysis. Identification of tylosin, spiramycin, virginiamycin, carbadox and olaquindox at sub-additive levels in compound feed. Confirmatory analysis by LC-MS

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

03-01-2018

Publisher

British Standards Institution

This European Standard specifies a high performance liquid chromatography – mass spectrometry (LC-MS/MS) method for the identification of tylosin, spiramycin, virginiamycin, carbadox and olaquindox in animal feeds.

This European Standard specifies a high performance liquid chromatography - mass spectrometry (LC-MS/MS) method for the identification of tylosin, spiramycin, virginiamycin, carbadox and olaquindox in animal feeds. The method is suitable for the identification of low concentrations of tylosin, spiramycin, virginiamycin, carbadox and olaquindox in compound animal feeds. A limit of identification of 1 mg/kg for tylosin, spiramycin and virginiamycin, 4 mg/kg for carbadox and 3 mg/kg for olaquindox should be obtained by using the described method. The method was fully validated during a collaborative study (see Annex A). Since tylosin, spiramycin and virginiamycin are fermentation products consisting of a mixture of several closely related compounds, the analysis is based on detection and identification of the most abundant constituents. For tylosin the marker is tylosin A, for spiramycin the marker is spiramycin I and II and for virginiamycin the marker is virginiamycin M1 and S1. The other isomers and forms can be readily detected with the same method but adjustment of the MS parameters according to the molecular mass of precursor and product ions need to be made. Carbadox and olaquindox are analysed as such.

Committee
AW/10
DocumentType
Standard
ISBN
9780580917950
Pages
34
PublisherName
British Standards Institution
Status
Current

Standards Relationship
EN 17049:2018 Identical

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Hardcopy - English
PDF - English
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