BS ISO 21149 : 2006
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
Hardcopy , PDF
01-31-2010
English
01-01-2006
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Plate count
4.3 Membrane filtration
4.4 Detection of bacteria by enrichment
5 Diluents, neutralizers and culture media
5.1 General
5.2 Neutralizing diluents and diluents
5.3 Diluent for the bacterial suspension (tryptone sodium
chloride solution)
5.4 Culture media
6 Apparatus and glassware
7 Strains of microorganisms
8 Handling of cosmetic products and laboratory samples
9 Procedure
9.1 General recommendation
9.2 Preparation of the initial suspension
9.3 Counting methods
9.4 Enrichment
10 Counting of colonies (plate counts and membrane filtration
methods)
11 Detection of growth (enrichment method)
12 Expression of results
12.1 Method of calculation for plate count
12.2 Interpretation
12.3 Examples
12.4 Detection after enrichment
13 Neutralization of the antimicrobial properties of the product
13.1 General
13.2 Preparation of inoculum
13.3 Validation of counting methods
13.4 Validation of the detection method by enrichment
13.5 Interpretation of validation results
14 Test report
Annex A (informative) Other neutralizing diluents
Annex B (informative) Other diluents
Annex C (informative) Other culture media
Annex D (informative) Neutralizers of antimicrobial activity of
preservatives and rinsing liquids
Bibliography
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