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PD CEN ISO/TS 18867:2015

Current
Current

The latest, up-to-date edition.

Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

03-31-2016

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principles
5 Reagents
6 Apparatus and equipment
7 Sampling
8 Procedure
9 Test report
Annex A (normative) - PCR detection and isolation of
        pathogenic Y. enterocolitica
Annex B (informative) - Real-time PCR for detection
        of Y. enterocolitica
Annex C (informative) - Detection and isolation of
        Y. pseudotuberculosis
Annex D (informative) - Simultaneous detection of
        pathogenic Y. enterocolitica and Y. pseudotuberculosis
        using multiplex real-time PCR
Bibliography

Defines two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods.

This Technical Specification specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real-time PCR-based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will be detected by the same primer/probe set as Y. pseudotuberculosis. However, Y. pestis is normally not associated with food. This Technical Specification is applicable to products for human consumption, animal feeding stuffs, and environmental samples.

Committee
AW/9
DocumentType
Standard
Pages
42
PublisherName
British Standards Institution
Status
Current

Standards Relationship
ISO/TS 18867:2015 Identical
CEN ISO/TS 18867:2015 Identical

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 6887-1:2017 Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 20837:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 10273:2017 Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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