CLSI M11 A7 : 7ED 2007
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
METHODS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ANAEROBIC BACTERIA
02-29-2012
01-12-2013
Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests on
Anaerobic Bacteria
5.1 Routine Testing
5.2 Surveillance Testing
6 Limitations of Susceptibility Testing of Anaerobic Bacteria
6.1 Interpretive Criteria (Breakpoints) and Clinical
Correlation
6.2 Organisms
7 Antimicrobial Agents
7.1 Source
7.2 Weighing Antimicrobial Powders
7.3 Preparing Stock Solutions
7.4 Number of Concentrations Tested
8 Selection of Antimicrobial Agents for Routine Testing and
Reporting
8.1 Routine Reports
8.2 Nonproprietary Names
9 Storage and Recovery of Isolates for Testing
9.1 Freezing Isolates for Later Testing
9.2 Recovering Isolates From Frozen Storage
10 Inoculum Preparation for Dilution Tests
10.1 Turbidity Standard for Inoculum Preparation
10.2 Growth Method
10.3 Direct Colony Suspension Method
11 Beta-lactamase Testing
12 Reference Agar Dilution Procedure (Wadsworth Method)
12.1 Reagents and Materials
12.2 Procedure for Preparing Agar Dilution Plates
12.3 Inoculation of Agar Dilution Plates
12.4 Incubation of Agar Dilution Plates
12.5 Reading Agar Dilution Plates
13 Broth Microdilution Procedure
13.1 Reagents and Materials
13.2 Procedure for Preparing Broth Microdilution Plates
13.3 Inoculation of Broth Microdilution Trays
13.4 Incubation of Broth Microdilution Trays
13.5 Reading Broth Microdilution Endpoints
14 Quality Control Guidelines
14.1 Purpose
14.2 Control Organisms and Frequency of Testing
14.3 Corrective Action
References
Appendix A. Preparation of Media and Supplements
Appendix B. Media, Reagents, Supplies, Equipment, and Timetable
for Agar Dilution Susceptibility Testing of Anaerobic
Bacteria
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