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CLSI MM18 A : 1ED 2008

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

INTERPRETIVE CRITERIA FOR IDENTIFICATION OF BACTERIA AND FUNGI BY DNA TARGET SEQUENCING

Superseded date

03-27-2023

Published date

01-12-2013

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Standard Precautions
4 Terminology
  4.1 Definitions
  4.2 Abbreviations and Acronyms
5 Approach to DNA Target Sequencing
  5.1 Primer Design
  5.2 Controls
  5.3 Protocol and Instrument Requirements
  5.4 Overview of Sequence Data
  5.5 Selection of Reference Databases
  5.6 Sequence Comparison for Identification
  5.7 Generalized Approach to Sequence-Based Identification
       for Bacteria (excluding Mycobacteria and Actinomycetes)
6 Interpretive Criteria of Identity Scores
  6.1 Staphylococci and Related Gram-Positive Cocci
  6.2 Streptococcus spp., Enterococcus spp., and
       Streptococcal-Like Organisms
  6.3 Glucose Nonfermenting Gram-Negative Bacilli
  6.4 Campylobacterales
  6.5 Gram-Positive Anaerobes
  6.6 Gram-Negative Anaerobes
  6.7 Coryneform Gram-Positive Bacilli
  6.8 Aerobic Actinomycetes
  6.9 Mycobacterium sp.
  6.10 Bacterial Agents of Bioterrorism
  6.11 Fungi
7 Suggestions for Result Reporting
References
Summary of Delegate Comments and Committee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Specifies recommendations for clinical laboratories that employ amplification and Sangerbased (dideoxy-termination) sequencing of broad-range DNA targets for the identification of bacteria, mycobacteria, and fungi from cultured clinical isolates.

DevelopmentNote
Supersedes NCCLS MM18 P. (04/2008)
DocumentType
Miscellaneous Product
PublisherName
Clinical Laboratory Standards Institute
Status
Superseded
SupersededBy
Supersedes

CLSI MM9 A2 : 2ED 2014 NUCLEIC ACID SEQUENCING METHODS IN DIAGNOSTIC LABORATORY MEDICINE
CLSI MM1 A3 : 3ED 2012 MOLECULAR METHODS FOR CLINICAL GENETICS AND ONCOLOGY TESTING
CLSI M52 : 1ED 2015 Verification of Commercial Microbial Identification and Antimicrobial Susceptibility Testing Systems, 1st Edition
CLSI MM19 A : 1ED 2011 ESTABLISHING MOLECULAR TESTING IN CLINICAL LABORATORY ENVIRONMENTS

CLSI MM10 A : 1ED 2006 GENOTYPING FOR INFECTIOUS DISEASES: IDENTIFICATION AND CHARACTERIZATION
CLSI M29 A3 : 3ED 2005 ACQUIRED INFECTIONS; APPROVED GUIDELINE
CLSI MM9 A : 1ED 2004 NUCLEIC ACID SEQUENCING METHODS IN DIAGNOSTIC LABORATORY MEDICINE
CLSI MM3 A2 : 2ED 2006 MOLECULAR DIAGNOSTIC METHODS FOR INFECTIOUS DISEASES

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