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DD ISO/TS 21872-1:2007

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Microbiology of food and animal feeding stuffs. Horizontal method for the detection of potentially enteropathogenic Vibrio spp. Detection of Vibrio parahaemolyticus and Vibrio cholerae
Available format(s)

Hardcopy , PDF

Superseded date

09-30-2017

Language(s)

English

Published date

12-31-2008

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 First enrichment in a liquid selective
        medium
   4.3 Second enrichment in a liquid selective
        medium
   4.4 Isolation and identification
   4.5 Confirmation
5 Culture media and reagents
   5.1 Enrichment medium: Alkaline saline
        peptone water (ASPW)
   5.2 Solid selective isolation media
   5.3 Saline nutrient agar (SNA)
   5.4 Reagent for detection of oxidase
   5.5 Saline triple sugar iron (TSI) agar
   5.6 Saline medium for detection of ornithine
        decarboxylase (ODC)
   5.7 Saline medium for detection of lysine
        decarboxylase (LDC)
   5.8 Saline medium for detection of arginine
        dihydrolase (ADH)
   5.9 Reagent for detection of beta-galactosidase
   5.10 Saline medium for detection of indole
   5.11 Saline peptone waters
   5.12 Sodium chloride solution
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
   9.1 Test portion and initial suspension
   9.2 First selective enrichment
   9.3 Second selective enrichment
   9.4 Isolation and identification
   9.5 Confirmation
10 Expression of results
11 Test report
Annex A (normative) Diagram of procedure
Annex B (normative) Composition and preparation of
        the culture media and reagents
Bibliography

Defines a horizontal method for the detection of the two main pathogenic Vibrio species causing intestinal illness in humans: V. parahaemolyticus and V. cholerae.

Committee
AW/9
DevelopmentNote
Supersedes BS 5763-14(1991). (03/2010)
DocumentType
Standard
Pages
28
PublisherName
British Standards Institution
RevisionOf
Status
Superseded
SupersededBy
Supersedes

Standards Relationship
ISO/TS 21872-1:2007 Identical

ISO/TS 11133-1:2009 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media
ISO 8261:2001 Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination

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