Name: DD ISO/TS 21872-2 : DRAFT DEC 2007
Brand: BSI

MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO SPP. - PART 2: DETECTION OF SPECIES OTHER THAN VIBRIO PARAHAEMOLYTICUS AND VIBRIO CHOLERAE

Published date

11-23-2012

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 First enrichment in a liquid selective medium
   4.3 Second enrichment in a liquid selective medium
   4.4 Isolation and identification
   4.5 Confirmation
5 Culture media, reagents
   5.1 Enrichment medium: Alkaline saline peptone
        water (ASPW)
   5.2 Solid selective isolation media
   5.3 Saline nutrient agar (SNA)
   5.4 Reagent for detection of oxidase
   5.5 Saline triple sugar iron (TSI) agar
   5.6 Saline medium for detection of ornithine
        decarboxylase (ODC)
   5.7 Saline medium for detection of lysine
        decarboxylase (LDC)
   5.8 Saline medium for detection of arginine
        dihydrolase (ADH)
   5.9 Reagent for detection of beta-galactosidase
   5.10 Saline medium for detection of indole
   5.11 Saline peptone waters
   5.12 Sodium chloride solution
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
   9.1 Test portion and initial suspension
   9.2 First selective enrichment
   9.3 Second selective enrichment
   9.4 Isolation and identification
   9.5 Confirmation
10 Expression of results
11 Test report
Annex A (normative) Diagram of procedure
Annex B (normative) Composition and preparation of
        the culture media and reagents
Bibliography

Defines a horizontal method for detection of the enteropathogenic Vibrio species, causing illness in or via the intestinal tract, other than Vibrio parahaemolyticus and Vibrio cholerae.

Committee	AW/9
DocumentType	Draft
PublisherName	British Standards Institution
Status	Current

Standards	Relationship
ISO/TS 21872-2:2007	Identical

ISO/TS 11133-1:2009	Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO/TS 11133-2:2003	Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media
ISO 8261:2001	Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination