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CEN
EN 14663:2005

EN 14663:2005

Current

Current

The latest, up-to-date edition.

Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC

Published date

12-07-2005

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) Precision data
Annex B (informative) Examples for suitable HPLC-conditions
                      for the determination of vitamin B[6]
                      compounds
Annex C (informative) Examples for molar extinction coefficients
Annex D (informative) Figures
Bibliography

This document specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC).Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives as well as the b-glycosylated forms that have been added to foodstuffs, calculated as pyridoxine.This method has been successfully validated with semolina with milk (infant food), potato puree, vegetables with ham (convenient products) and a multi vitamin drink at levels from 0,034 mg/100 g to 1,21 mg/100 g.

Committee
CEN/TC 275
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current

Standards Relationship
NF EN 14663 : 2006 Identical
NS EN 14663 : 1ED 2006 Identical
DIN EN 14663 E : 2006 Identical
SN EN 14663 : 2006 Identical
UNE-EN 14663:2007 Identical
I.S. EN 14663:2006 Identical
UNI EN 14663 : 2006 Identical
NBN EN 14663 : 2006 Identical
NEN EN 14663 : 2005 Identical
BS EN 14663:2005 Identical
PN EN 14663 : SEP 2006 Identical
DIN EN 14663:2006-03 Identical
UNE-EN 14663:2005 Identical

DIN EN 14164:2014-08 FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HIGH PERFORMANCE CHROMATOGRAPHY
07/30163680 DC : 0 BS EN 14164 - FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HPLC
PREN 14164 : DRAFT 2012 FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HPLC
12/30258767 DC : 0 BS EN 14164 - FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HPLC
I.S. EN 14164:2014 FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HIGH PERFORMANCE CHROMATOGRAPHY
BS EN 14164:2014 Foodstuffs. Determination of vitamin B6 by high performance chromatography
UNE-EN 14164:2014 Foodstuffs - Determination of vitamin B6 by high performance chromatography
UNI EN 14164 : 2014 FOODSTUFFS - DETERMINATION OF VITAMIN B6 BY HIGH PERFORMANCE CHROMATOGRAPHY
EN 14164:2014 Foodstuffs - Determination of vitamin B6 by high performance chromatography

ISO 5725:1986 Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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