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EN ISO 11731:2017

Current

Current

The latest, up-to-date edition.

Water quality - Enumeration of Legionella (ISO 11731:2017)

Published date

06-07-2017

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus and glassware
6 Culture media and reagents
7 Sampling
8 Procedure
9 Expression of results
10 Test report
11 Quality assurance
Annex A (informative) - Legionella species
Annex B (normative) - Culture media
Annex C (normative) - Diluents
Annex D (normative) - Acid solution
Annex E (informative) - Scraping or rubbing the
        bacteria from membrane filters
Annex F (informative) - Centrifugation technique
Annex G (informative) - Indirect immunofluorescent
        antibody assay for the identification of Legionella
        species
Annex H (informative) - Performance data
Annex I (informative) - Pre-treatment of water related
        matrices
Annex J (normative) - Decision matrix
Bibliography

ISO 11731:2017 specifies culture methods for the isolation of Legionella and estimation of their numbers in water samples.These methods are applicable to all kinds of water samples including potable, industrial, waste and natural waters. These methods can be used for water related matrices, e.g. biofilms, sediments, etc.Not all Legionella species are culturable; therefore, the methods described in this document do not recover all species of Legionella.

Committee
CEN/TC 230
DevelopmentNote
Supersedes EN ISO 11731-2. (06/2017)
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Current
Supersedes

17/30362689 DC : 0 BS EN 16941-2 - ON-SITE NON-POTABLE WATER SYSTEMS - PART 2: SYSTEMS FOR THE USE OF TREATED GREYWATER

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 7704:1985 Water quality — Evaluation of membrane filters used for microbiological analyses
ISO 19458:2006 Water quality Sampling for microbiological analysis
ISO 8199:2005 Water quality General guidance on the enumeration of micro-organisms by culture
ISO 13843:2017 Water quality — Requirements for establishing performance characteristics of quantitative microbiological methods
ISO 11133:2014 Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods

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