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EN ISO 15216-1:2017

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)
Superseded date

04-30-2021

Published date

03-29-2017

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation of reagents
        and buffers
Annex C (informative) - Real-time RT-PCR mastermixes and
        cycling parameters
Annex D (informative) - Real-time RT-PCR primers and
        hydrolysis probes for the detection of HAV, norovirus
        GI and GII and mengo virus (process control)
Annex E (informative) - Growth of mengo virus strain MC[0]
        for use as a process control
Annex F (informative) - RNA extraction using the NucliSENS[R]
        system
Annex G (informative) - Generation of dsDNA control stocks
Annex H (informative) - Generation of EC RNA stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Method validation studies and
        performance characteristics
Bibliography

ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

Committee
CEN/TC 463
DevelopmentNote
Supersedes CEN ISO/TS 15216-1. (04/2017)
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Superseded
SupersededBy
Supersedes

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 17468:2016 Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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