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EN ISO 15952:2011

Superseded
Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Soil quality - Effects of pollutants on juvenile land snails (Helicidae) - Determination of the effects on growth by soil contamination (ISO 15952:2006)
Superseded date

07-01-2019

Published date

06-22-2011

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents
7 Apparatus
8 Storage and preparation of the samples
9 Procedure
10 Reference substance
11 Calculations and expression of results
12 Validity of test for Helix aspersa aspersa
13 Test report
Annex A (normative) - Static method
Annex B (informative) - Breeding technique for snails
Annex C (informative) - Example of composition of
        snail feed
Annex D (informative) Example of table of data
Annex E (informative) Example of results with Helix
        aspersa aspersa
Annex F (informative) Determination of the effects on
        growth by food contamination
Annex G (informative) Test performance with other
        snail species
Bibliography

ISO 15952:2005 specifies a semi-static method for the determination of the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to which defined amounts of the following are added: substances or preparations; and soils (contaminated or of unknown quality) or waste materials. A static method is also described.ISO 15952:2005 does not apply to volatile substances, i.e. substances for which the Henry constant, H, or the air/water partition coefficient is over 1, or for which the vapour pressure is over 0,013 3 Pa at 25 °C.This test takes into account possible changes in the test substance, preparation, soil or waste material because the test mixture is prepared and renewed every 7 days during the 28-day test period.

Committee
CEN/TC 444
DocumentType
Standard
PublisherName
Comite Europeen de Normalisation
Status
Superseded
SupersededBy

NFX 31 260 : 1999 SOIL QUALITY - EFFECTS OF POLLUTANTS ON INSECT LARVAE (OXYTHYREA FUNESTA) - DETERMINATION OF ACUTE TOXICITY USING ARTIFICIAL SOIL SUBSTRATE
ISO 11269-2:2012 Soil quality — Determination of the effects of pollutants on soil flora — Part 2: Effects of contaminated soil on the emergence and early growth of higher plants
ISO 10381-6:2009 Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
ISO 16387:2014 Soil quality — Effects of contaminants on Enchytraeidae (Enchytraeus sp.) — Determination of effects on reproduction
EN 14735:2005/AC:2006 CHARACTERIZATION OF WASTE - PREPARATION OF WASTE SAMPLES FOR ECOTOXICITY TESTS
ISO 11267:2014 Soil quality — Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants
NFX 31 255-2 : 2001 SOIL QUALITY - EFFECTS OF CONTAMINANTS ON JUVENILE LAND SNAILS (HELIX ASPERSA) - PART 2: DETERMINATION OF THE EFFECTS ON GROWTH BY SOIL CONTAMINATION
ISO 11268-2:2012 Soil quality — Effects of pollutants on earthworms — Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei
ISO 11274:1998 Soil quality Determination of the water-retention characteristic Laboratory methods
ISO 10390:2005 Soil quality Determination of pH
ISO 10694:1995 Soil quality Determination of organic and total carbon after dry combustion (elementary analysis)
ISO/TS 20281:2006 Water quality — Guidance on statistical interpretation of ecotoxicity data
ISO 11268-1:2012 Soil quality — Effects of pollutants on earthworms — Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andrei
ISO 11260:1994 Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solution

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