Name: EN ISO 20838:2006
Brand: CEN

Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods (ISO 20838:2006)

Published date

04-15-2006

Publisher

Comite Europeen de Normalisation

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and equipment
7 Procedure
8 Interpretation
9 Performance
10 Test report
Bibliography

ISO 20838:2006 provides the overall framework for qualitative methods for the detection of food-borne pathogens using the polymerase chain reaction (PCR). It covers the general requirements for the specific amplification of target nucleic acid sequences and the detection and confirmation of the identity of the amplified nucleic acid sequence. Guidelines, minimum requirements and performance characteristics described in ISO 20838:2006 are intended to ensure that comparable and reproducible results are obtained in different laboratories.ISO 20838:2006 has been established for food-borne pathogens in or isolated from food and feed matrices, but can also be applied to other matrices, for example environmental samples, or to the detection of other microorganisms under investigation.

Committee	CEN/TC 463
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
ISO 20838:2006	Identical
PN EN ISO 20838 : 2007	Identical
I.S. EN ISO 20838:2006	Identical
NS EN ISO 20838 : 1ED 2006	Identical
SS-EN ISO 20838 : 2006	Identical
NEN EN ISO 20838 : 2006	Identical
NF EN ISO 20838 : 2006	Identical
SN EN ISO 20838 : 2006	Identical
UNE-EN ISO 20838:2007	Identical
DIN EN ISO 20838 E : 2006	Identical
BS EN ISO 20838:2006	Identical
NBN EN ISO 20838 : 2006	Identical
UNI EN ISO 20838 : 2006	Identical
UNE-EN ISO 20838:2006	Identical
DIN EN ISO 20838:2006-08	Identical

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO/TS 20836:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 20837:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 16140:2003	Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods