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I.S. EN 12821:2009

Current
Current

The latest, up-to-date edition.

FOODSTUFFS - DETERMINATION OF VITAMIN D BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY - MEASUREMENT OF CHOLECALCIFEROL (D[3]) OR ERGOCALCIFEROL (D[2])
Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-01-2009

Preview

For Harmonized Standards, check the EU site to confirm that the Standard is cited in the Official Journal.

Only cited Standards give presumption of conformance to New Approach Directives/Regulations.

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of suitable HPLC
        systems
Annex B (informative) - Examples of suitable extraction
        and saponification conditions
Annex C (normative) - Examples of suitable semi-preparative
        and analytical HPLC chromatograms
Annex D (informative) - Precision data
Annex E (informative) - Additional cleanup step for the
        determination of vitamin D with use of preparative
        TLC, column chromatography and or SPE
Bibliography

Describes a method for the determination of vitamin D[3] (cholecalciferol) or vitamin D[2] (ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC).

DocumentType
Standard
Pages
26
PublisherName
National Standards Authority of Ireland
Status
Current
Supersedes

Standards Relationship
DIN EN 12821:2009-08 Identical
NEN EN 12821 : 2009 Identical
UNE-EN 12821:2009 Identical
EN 12821:2009 Identical
BS EN 12821:2009 Identical
UNI EN 12821 : 2009 Identical
NF EN 12821 : 2009 Identical
NBN EN 12821 : 2009 Identical
NS EN 12821 : 2009 Identical
SN EN 12821 : 2009 Identical

ISO 5725:1986 Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)

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