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ISO 20837:2006

Current
Current

The latest, up-to-date edition.

Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
Available format(s)

Hardcopy , PDF , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English, French, Russian

Published date

04-06-2006

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.

It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.

ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

DevelopmentNote
Supersedes ISO/DIS 20837 (04/2006)
DocumentType
Standard
Pages
7
PublisherName
International Organization for Standardization
Status
Current

S.R. CEN ISO/TS 18867:2015 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF PATHOGENIC YERSINIA ENTEROCOLITICA AND YERSINIA PSEUDOTUBERCULOSIS (ISO/DTS 18867:2015)
UNI CEN ISO/TS 17919 : 2013 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF BOTULINUM TYPE A, B, E AND F NEUROTOXIN-PRODUCING CLOSTRIDIA
ISO 21872-1:2017 Microbiology of the food chain — Horizontal method for the determination of Vibrio spp. — Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
CEN ISO/TS 18867:2015 Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis (ISO/TS 18867:2015)
BS EN ISO 22119:2011 Microbiology of food and animal feeding stuffs. Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions
UNI CEN ISO/TS 13136 : 2013 MICROBIOLOGY OF FOOD AND ANIMAL FEED - REAL-TIME POLYMERASE CHAIN REACTION (PCR)-BASED METHOD FOR THE DETECTION OF FOODBORNE PATHOGENS - HORIZONTAL METHOD FOR THE DETECTION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) AND THE DETERMINATION OF O157, O111, O26, O103 AND O145 SEROGROUPS
PD CEN ISO/TS 17919:2013 Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
I.S. EN ISO 22119:2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - REAL-TIME POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
PD CEN ISO/TS 18867:2015 Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis
UNE-EN ISO 22119:2011 Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)
S.R. CEN ISO/TS 17919:2013 MICROBIOLOGY OF THE FOOD CHAIN - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - DETECTION OF BOTULINUM TYPE A, B, E AND F NEUROTOXIN-PRODUCING CLOSTRIDIA (ISO/TS 17919:2013)
DIN EN ISO 22174:2005-05 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
ISO/TS 17919:2013 Microbiology of the food chain Polymerase chain reaction (PCR) for the detection of food-borne pathogens Detection of botulinum type A, B, E and F neurotoxin-producing clostridia
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
DIN EN ISO 21872-1:2017-10 Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
07/30171460 DC : 0 BS ISO 22119 - MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - REAL-TIME POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
DIN EN ISO 21872-1 E : 2017 MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE DETERMINATION OF VIBRIO SPP. - PART 1: DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO PARAHAEMOLYTICUS, VIBRIO CHOLERAE AND VIBRIO VULNIFICUS (ISO 21872-1:2017)
ISO/TS 18867:2015 Microbiology of the food chain — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis
EN ISO 22119:2011 Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)
UNI EN ISO 22119 : 2011 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - REAL-TIME POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
PD CEN ISO/TS 13136:2012 Microbiology of food and animal feed. Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens. Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
DIN EN ISO 22119:2011-11 Microbiology of food and animal feeding stuffs - Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22119:2011)
BS EN ISO 20838:2006 Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Requirements for amplification and detection for qualitative methods
ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO/TS 13136:2012 Microbiology of food and animal feed — Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens — Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups
ISO 22119:2011 Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
EN ISO 22174:2005 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - General requirements and definitions (ISO 22174:2005)
EN ISO 20838:2006 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods (ISO 20838:2006)
EN ISO 21872-1:2017 Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)
BS EN ISO 21872-1:2017 Microbiology of the food chain. Horizontal method for the determination of Vibrio spp. Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus
S.R. CEN ISO/TS 13136:2012 MICROBIOLOGY OF FOOD AND ANIMAL FEED - REAL-TIME POLYMERASE CHAIN REACTION (PCR)-BASED METHOD FOR THE DETECTION OF FOOD-BORNE PATHOGENS - HORIZONTAL METHOD FOR THE DETECTION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) AND THE DETERMINATION OF O157, O111, O26, O103 AND O145 SEROGROUPS (ISO/TS 13136:2012)
I.S. EN ISO 21872-1:2017 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE DETECTION OF POTENTIALLY ENTEROPATHOGENIC VIBRIO SPP. - PART 1: DETECTION OF VIBRIO PARAHAEMOLYTICUS AND VIBRIO CHOLERAE (ISO 21872-1:2017)
DIN EN ISO 20838 E : 2006 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - REQUIREMENTS FOR AMPLIFICATION AND DETECTION FOR QUALITATIVE METHODS
BS EN ISO 22174:2005 Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions
CEN ISO/TS 13136:2012 Microbiology of food and animal feed - Real-time polymerase chain reaction (PCR)-based method for the detection of food-borne pathogens - Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) and the determination of O157, O111, O26, O103 and O145 serogroups (ISO/TS 13136:2012)
DIN EN ISO 20838:2006-08 Microbiology of food and animal feeding stuffs - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Requirements for amplification and detection for qualitative methods (ISO 20838:2006)
CEN ISO/TS 17919:2013 Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin-producing clostridia (ISO/TS 17919:2013)

ISO 22174:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 6579:2002 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Salmonella spp.
ISO 21567:2004 Microbiology of food and animal feeding stuffs Horizontal method for the detection of Shigella spp.
ISO 11290-1:2017 Microbiology of the food chain — Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method
ISO/TS 20836:2005 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 7937:2004 Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of Clostridium perfringens — Colony-count technique
ISO 10272-1:2017 Microbiology of the food chain — Horizontal method for detection and enumeration of Campylobacter spp. — Part 1: Detection method
ISO 6888-3:2003 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 3: Detection and MPN technique for low numbers
ISO 7932:2004 Microbiology of food and animal feeding stuffs Horizontal method for the enumeration of presumptive Bacillus cereus Colony-count technique at 30 degrees C
ISO 10273:2017 Microbiology of the food chain — Horizontal method for the detection of pathogenic Yersinia enterocolitica
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 16654:2001 Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157
DIN 10135:2013-05 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - METHOD FOR THE DETECTION OF SALMONELLA

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