NEN EN 46-2 : 2016

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test materials
   5.1 Biological material
       5.1.1 Hylotrupes bajulus (Linnaeus) females
       5.1.2 Source of females
   5.2 Products and reagents
   5.3 Apparatus
       5.3.1 Culturing chamber, with air circulation, and
             controlled at (28 +/- 2) degree C and at relative
             humidity (70 +/- 5) %
       5.3.4 Testing chamber, ventilated and air conditioned.
             controlled at (22 +/- 2) degree C and at relative
             humidity (70 +/- 5) %
6 Sampling
7 Test specimens
   7.1 Species of wood
   7.2 Wood quality
   7.3 Provision of test specimens
   7.4 Dimensions of test specimens
   7.5 Number of test specimens
       7.5.1 Test specimens of egg-laying
       7.5.2 Test specimens for checking the tunnelling ability
             and the morality of the larvae
8 Procedure
   8.1 Preparation of the test specimens
       8.1.1 Conditioning of the test specimens prior to sealing
       8.1.2 Sealing of the transverse faces
       8.1.3 Treatment of the test specimens
       8.1.4 Drying and conditioning of the test specimens after
         treatment
   8.2 Exposure of the test specimens to the insects
       (ovicidal action)
   8.3 Validity of test
9 Expression of results
   9.1 Ovicidal test
   9.2 Tunnelling control
   9.3 Total morality
10 Test report
Annex A (informative) Example of a test report
Hatching ability
Tunnelling ability
Annex B (informative) Technique for culturing Hylotrupes
                      bajulus (Linnaeus)
Annex C (informative) Environmental, health and safety
                      precautions within Chemical/biological
                      laboratory
Bibliography