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NEN EN ISO 10705-2 : 2001

Current

Current

The latest, up-to-date edition.

WATER QUALITY - DETECTION AND ENUMERATION OF BACTERIOPHAGES - PART 2: ENUMERATION OF SOMATIC COLIPHAGES

Published date

01-12-2013

1 Scope
2 Normative references
3 Terms and definitions
4 Safety precautions
5 Principle
6 Diluent, culture media and reagents
   6.1 Basic materials
   6.2 Diluent
7 Apparatus and glassware
8 Microbiological reference cultures
9 Sampling
10 Preparation of test material
   10.1 Culturing and maintenance of host strains
   10.2 Calibration of absorbance measurements for
        counts of viable microorganisms
11 Procedure
   11.1 Preparation of inoculum cultures
   11.2 Standard procedure
   11.3 Method for samples with high bacterial
        background flora
   11.4 Samples with low phage counts
   11.5 Presence/absence test
   11.6 Quality assurance
12 Expression of results
   12.1 Plaque count procedures (11.2 to 11.4)
   12.2 Presence/absence test (11.5)
13 Test report
Annex A (normative) Culture media, reagents and
        diluent
Annex B (informative) General description of
        somatic bacteriophages
Annex C (informative) Culturing of bacteriaophage
        X174
Bibliography

Describes a method for the detection and enumeration of somatic coliphages by incubating the sample with an appropriate host strain. Applicable to all kinds of water, sediments and sludge extracts, where necessary after dilution.

DevelopmentNote
Supersedes NEN ISO 10705-2. (10/2002)
DocumentType
Standard
PublisherName
Netherlands Standards
Status
Current
Supersedes

Standards Relationship
UNI EN ISO 10705-2 : 2001 Identical
DIN EN ISO 10705-2:2002-01 Identical
ISO 10705-2:2000 Identical
UNE-EN ISO 10705-2:2002 Identical
BS EN ISO 10705-2:2001 Identical
EN ISO 10705-2:2001 Identical

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