NEN EN ISO 22174 : 2005
Current
The latest, up-to-date edition.
MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - GENERAL REQUIREMENTS AND DEFINITIONS
01-12-2013
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 General
4.2 Preliminary microbial enrichment
4.3 Nucleic acid preparation
4.4 PCR amplification
4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
6.1 General
6.2 Personnel
6.3 Laboratory setup
6.4 Waste management
7 Reagents
8 Apparatus and equipment
8.1 General
8.2 Special considerations
9 Procedure
9.1 Sample preparation
9.2 Amplification
9.3 Control reaction
9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography
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