PREN 16418 : DRAFT 2012

Name: PREN 16418 : DRAFT 2012
Brand: CEN

Superseded

View Superseded by

PAPER AND BOARD - DETERMINATION OF THE CYTOTOXICITY OF AQUEOUS EXTRACTS USING A METABOLICALLY COMPETENT HEPATOMA CELL LINE (HEPG2)

Superseded date

04-01-2014

Published date

01-12-2013

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Cell line
7 Food simulants used for testing
8 Cleaning laboratory glassware
9 Equipment
10 Preparation of specimens
11 Cytotoxicity assessment
12 Expression of the results
13 Interpretation of the results
14 Precision
15 Test report
Annex A (informative) - 96-well plates configuration
Annex B (informative) - RNA Synthesis rate inhibition
cytotoxicity test work flow
Annex C (informative) - Validation of the two methods
(option A and B)
Bibliography

Defines a test method for the laboratory assessment of the potential cytotoxic effect of paper and board food contact materials using specifically the HepG2 cell line.

Committee	TC 172
DocumentType	Draft
PublisherName	Comite Europeen de Normalisation
Status	Superseded
SupersededBy	EN 16418:2014

Standards	Relationship
12/30259424 DC : 0	Identical
NEN EN 16418 : 2014	Identical

EN 645:1993	Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract
EN 15845:2010	Paper and board - Determination of the cytotoxicity of aqueous extracts
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 647:1993	Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract