MICROBIOLOGY OF FOOD AND ANIMAL FEED - REAL-TIME POLYMERASE CHAIN REACTION (PCR)-BASED METHOD FOR THE DETECTION OF FOOD-BORNE PATHOGENS - HORIZONTAL METHOD FOR THE DETECTION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC) AND THE DETERMINATION OF O157, O111, O26, O103 AND O145 SEROGROUPS (ISO/TS 13136:2012)

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Hardcopy , PDF

Language(s)

English

Published date

01-01-2012

Publisher

National Standards Authority of Ireland

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Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluents, culture media and reagents
6 Equipment
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Performance data
Annex A (normative) - Flow diagram of the screening
        procedure
Annex B (normative) - Flow diagram of the isolation
        and confirmation procedure
Annex C (informative) - Identification of Shiga
        toxin-producing Escherichia coli (STEC)
        by multiplex PCR amplification of virulence
        genes and detection of PCR products by
        agarose gel electrophoresis
Annex D (informative) - Internal amplification control
Annex E (informative) - Primers and probes for the
        PCR assays
Annex F (normative) - Isolation of STEC strains
Bibliography

Specifies the identification of Shiga toxin-producing Escherichia coli (STEC) by means of the detection of the following genes: a) the major virulence genes of STEC, stx and eae; b) the genes associated with the serogroups O157, O111, O26, O103, and O145.

DocumentType	Standard
Pages	37
PublisherName	National Standards Authority of Ireland
Status	Current

Standards	Relationship
CEN ISO/TS 13136:2012	Identical
ISO/TS 13136:2012	Identical

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 16140-2:2016	Microbiology of the food chain Method validation Part 2: Protocol for the validation of alternative (proprietary) methods against a reference method
ISO/TS 19036:2006	Microbiology of food and animal feeding stuffs Guidelines for the estimation of measurement uncertainty for quantitative determinations
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 7550:1985	Laboratory glassware — Disposable micropipettes
ISO 20837:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 16140:2003	Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods
ISO 16654:2001	Microbiology of food and animal feeding stuffs — Horizontal method for the detection of Escherichia coli O157

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S.R. CEN ISO/TS 13136:2012

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