Name: UNI EN 16418 : 2014
Brand: UNI

PAPER AND BOARD - DETERMINATION OF THE CYTOTOXICITY OF AQUEOUS EXTRACTS USING A METABOLICALLY COMPETENT HEPATOMA CELL LINE (HEPG[2])

Published date

05-08-2014

Publisher

Ente Nazionale Italiano di Unificazione (UNI)

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Cell line
7 Food simulants used for testing
8 Cleaning laboratory glassware
9 Equipment
10 Preparation of specimens
11 Cytotoxicity assessment
12 Expression of the results
13 Interpretation of the results
14 Precision
15 Test report
Annex A (informative) - 96-well plates configuration
Annex B (informative) - RNA Synthesis rate
inhibition cytotoxicity test work flow
Annex C (informative) - Validation of the two
methods (option A and B)
Bibliography

The standard specifies a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.

Committee	CT 8
DocumentType	Test Method
PublisherName	Ente Nazionale Italiano di Unificazione (UNI)
Status	Current

Standards	Relationship
EN 16418:2014	Identical

EN 645:1993	Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract
EN 15845:2010	Paper and board - Determination of the cytotoxicity of aqueous extracts
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
EN 647:1993	Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract