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UNI EN ISO 22959 : 2009

Current
Current

The latest, up-to-date edition.

ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS BY ON-LINE DONOR-ACCEPTOR COMPLEX CHROMATOGRAPHY AND HPLC WITH FLUORESCENCE DETECTION
Published date

08-26-2009

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents, materials and standards
6 Apparatus
7 Sampling and preparation of the test sample
8 Sample preparation
9 Procedure
   9.1 HPLC analysis
   9.2 Identification of PAHs
10 Calculation of individual PAHs
11 Method validation data
   11.1 Accuracy
   11.2 Within-laboratory precision
   11.3 Recovery
   11.4 Dynamic range
   11.5 Limit of quantification
12 Precision
   12.1 International collaborative trial
   12.2 Repeatability
   12.3 Reproducibility
13 Test report
Annex A (informative) - Example of the individual parts of
        an HPLC system
Annex B (informative) - Example of the operating conditions
        of the pumps of the HPLC system
Annex C (informative) - Example of the operating conditions
        of the column thermostat and the detector of the HPLC
        system
Annex D (informative) - Example of the autosampler
        programme of the HPLC system
Annex E (informative) - Tubing connections of the HPLC
        system
Annex F (informative) - Chromatogram of a standard
        calibration sample
Annex G (informative) - Determination precision for a
        sunflower oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex H (informative) - Determination precision for an olive
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex I (informative) - Determination precision for a soybean
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex J (informative) - Determination precision for a coconut
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex K (informative) - Interlaboratory test
Bibliography

Describes a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.

Committee
CT 3
DocumentType
Standard
PublisherName
Ente Nazionale Italiano di Unificazione (UNI)
Status
Current

Standards Relationship
ISO 22959:2009 Identical
EN ISO 22959:2009 Identical

ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 5555:2001 Animal and vegetable fats and oils — Sampling
ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 661:2003 Animal and vegetable fats and oils Preparation of test sample

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