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VDI 4253 Blatt 2:2004-06

Current
Current

The latest, up-to-date edition.

Measurement of airborne microorganisms and viruses in ambient air - Culture based method for the determination of the concentration of mould in air - Indirect method after sampling with gelatine/polycarbonate filters
Available format(s)

Hardcopy , PDF

Language(s)

German - English

Published date

06-01-2004

Preliminary note
Introduction
1 Scope
2 Terminology
3 Fundamentals for the detection of moulds
4 Quantitative assessment
5 Qualitative evidence/Identification
6 Documentation
Annex A - Compilation of biological parameters
          for moulds
Annex B - Sample exchange for method
          Validation
Annex C - Instructions for Analysis
Bibliography

In the document, the concentration of mesophilic and thermotolerant molds that can be cultivated on DG-18 agar, averaged over the sampling period, is determined by means of an indirect method after collection on a combination of gelatine filter with a polycarbonate filter underneath using a bioaerosol sampling tool based on VDI 2463 sheet 8 developed sampling head in the air (VDI 4252 Sheet 2).

DocumentType
Standard
Pages
32
PublisherName
Verlag des Vereins Deutscher Ingenieure
Status
Current
Supersedes

CEN/TS 16115-1:2011 Ambient air quality - Measurement of bioaerosols - Part 1: Determination of moulds using filter sampling systems and culture-based analyses
VDI 3475 Blatt 2:2005-12 Emission control - Facilities for biological waste - Composting an anaerobic (co-)digestion - Plant capacities up to approx. 6000 Mg/a
DIN ISO 12219-5:2014-10 INTERIOR AIR OF ROAD VEHICLES - PART 5: SCREENING METHOD FOR THE DETERMINATION OF THE EMISSIONS OF VOLATILE ORGANIC COMPOUNDS FROM VEHICLE INTERIOR PARTS AND MATERIALS - STATIC CHAMBER METHOD (ISO 12219-5:2014)
BS ISO 16000-16:2008 Indoor air Detection and enumeration of moulds. Sampling by filtration
DIN ISO 12219-4:2013-12 INTERIOR AIR OF ROAD VEHICLES - PART 4: METHOD FOR THE DETERMINATION OF THE EMISSIONS OF VOLATILE ORGANIC COMPOUNDS FROM VEHICLE INTERIOR PARTS AND MATERIALS - SMALL CHAMBER METHOD (ISO 12219-4:2013)
VDI 4250 Blatt 2:2015-11 Bioaerosols and biological agents - Environmental health assessment of bioaerosols in ambient air - Risk assessment for aerosols containing Legionella
VDI 4252 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Active sampling of bioaerosols - Separation of airborne mould on gelatine/polycarbonate filters
DIN ISO 16000-20:2015-11 INDOOR AIR - PART 20: DETECTION AND ENUMERATION OF MOULDS - DETERMINATION OF TOTAL SPORE COUNT (ISO 16000-20:2014)
DIN ISO 12219-6:2015-04 (Draft) INTERIOR AIR OF ROAD VEHICLES - PART 6: METHOD FOR THE DETERMINATION OF THE EMISSIONS OF SEMI-VOLATILE ORGANIC COMPOUNDS FROM VEHICLE INTERIOR PARTS AND MATERIALS AT HIGHER TEMPERATURE - SMALL CHAMBER METHOD (ISO 12219-6:2017)
VDI 4251-1 : 2007 COR 2 2016 MEASUREMENT OF AIRBORNE MICROORGANISMS AND VIRUSES IN AMBIENT AIR - PLANNING OF PLANT-RELATED AMBIENT AIR MEASUREMENTS - PLUME MEASUREMENT
DIN EN ISO 16000-32:2014-10 INDOOR AIR - PART 32: INVESTIGATION OF BUILDINGS FOR THE OCCURRENCE OF POLLUTANTS (ISO 16000-32:2014)
DIN EN ISO 16000-26:2012-11 Indoor air - Part 26: Sampling strategy for carbon dioxide (CO<(Index)2>) (ISO 16000-26:2012); German version EN ISO 16000-26:2012
DIN ISO 16000-18:2012-01 Indoor air - Part 18: Detection and enumeration of moulds - Sampling by impaction (ISO 16000-18:2011 + Cor.1:2011)
DIN EN ISO 16000-19:2014-12 Indoor air - Part 19: Sampling strategy for moulds (ISO 16000-19:2012)
VDI 4255 Blatt 2:2009-12 Bioaerosols and biological agents - Emission sources and control measures in livestock operations - Overview
VDI 4258 Blatt 1:2017-03 Bioaerosols and biological agents - Generation of test bioaerosols - Basics and requirements for test bioaerosols
VDI 4257 Blatt 2:2011-09 Bioaerosols and biological agents - Emission measurement - Sampling of bioaerosols and separation in liquids
DIN ISO 12219-3:2013-12 Interior air of road vehicles - Part 3: Screening method for the determination of the emissions of volatile organic compounds from vehicle interior parts and materials - Micro-scale chamber method (ISO 12219-3:2012)
UNE-CEN/TS 16115-1:2013 Ambient air quality - Measurement of bioaerosols - Part 1: Determination of moulds using filter sampling systems and culture-based analyses
DIN ISO 16000-31:2014-09 INDOOR AIR - PART 31: MEASUREMENT OF FLAME RETARDANTS AND PLASTICIZERS BASED ON ORGANOPHOSPHORUS COMPOUNDS - PHOSPHORIC ACID ESTER (ISO 16000-31:2014)
DIN ISO 16000-29:2015-02 INDOOR AIR - PART 29: TEST METHODS FOR VOC DETECTORS (ISO 16000-29:2014)
VDI 4251 Blatt 3:2015-08 Measurement of airborne microorganisms and viruses in ambient air - Plant-related dispersion modelling for bioaerosols
DIN ISO 12219-2:2012-11 Interior air of road vehicles - Part 2: Screening method for the determination of the emissions of volatile organic compounds from vehicle interior parts and materials - Bag method (ISO 12219-2:2012)
VDI 4253 Blatt 4:2013-02 Bioaerosols and biological agents - Determination of total cell count by fluorescence analysis after staining with DAPI
VDI 4255-1 : 2005 COR 2016 BIOAEROSOLS AND BIOLOGICAL AGENTS - SOURCES OF EMISSIONS AND CONTROL MEASURES - OVERVIEW
VDI 4255 Blatt 3:2016-12 Bioaerosols and biological agents - Emission factors for poultry
DIN ISO 16000-27:2014-11 INDOOR AIR - PART 27: DETERMINATION OF SETTLED FIBROUS DUST ON SURFACES BY SEM (SCANNING ELECTRON MICROSCOPY) (DIRECT METHOD) (ISO 16000-27:2014)
DIN ISO 16000-24:2013-01 INDOOR AIR - PART 24: PERFORMANCE TEST FOR EVALUATING THE REDUCTION OF VOLATILE ORGANIC COMPOUND (EXCEPT FORMALDEHYDE) CONCENTRATIONS BY SORPTIVE BUILDING MATERIALS (ISO 16000-24:2009)
DIN ISO 12219-1:2013-12 Interior air of road vehicles - Part 1: Whole vehicle test chamber - Specification and method for the determination of volatile organic compounds in cabin interiors (ISO 12219-1:2012)
DIN ISO 16000-6:2012-11 Indoor air - Part 6: Determination of volatile organic compounds in indoor and test chamber air by active sampling on Tenax TA? sorbent, thermal desorption and gas chromatography using MS or MS-FID (ISO 16000-6:2011)
DIN ISO 16000-25:2012-01 Indoor air - Part 25: Determination of the emission of semi-volatile organic compounds by building products - Micro-chamber method (ISO 16000-25:2011)
DIN ISO 16000-28:2012-12 Indoor air - Part 28: Determination of odour emissions from building products using test chambers (ISO 16000-28:2012)
VDI 4251 Blatt 2:2015-08 Measurement of airborne microorganisms and viruses in ambient air - Determination of area-specific background concentrations
DIN ISO 16000-4:2012-11 INDOOR AIR - PART 4: DETERMINATION OF FORMALDEHYDE - DIFFUSIVE SAMPLING METHOD (ISO 16000-4:2011)
DD CEN/TS 16115-1:2011 Ambient air quality. Measurement of bioaerosols Determination of moulds using filter sampling systems and culture-based analyses
VDI 3787 Blatt 10:2010-03 Environmental meteorology - Human biometeorological requirements in the framework of recreation, prevention, therapy, and rehabilitation
DIN ISO 16000-3:2013-01 Indoor air - Part 3: Determination of formaldehyde and other carbonyl compounds in indoor air and test chamber air - Active sampling method (ISO 16000-3:2011)
ISO 16000-16:2008 Indoor air — Part 16: Detection and enumeration of moulds — Sampling by filtration
VDI 4251 Blatt 4:2017-01 Measurement of airborne microorganisms and viruses in ambient air - Determination of existing load
VDI 3898:2013-01 Emission control - Plants for dry mechanical, physio-chemical, thermal and biological soil treatment
PD CEN/TS 16115-2:2016 Ambient air. Measurement of bioaerosols Planning and evaluation of plant-related plume measurements
VDI 4250 Blatt 1:2014-08 Bioaerosols and biological agents - Risk assessment of source-related ambient air measurements in the scope of environmental health - Effects of bioaerosol pollution on human health
DIN ISO 12219-7:2016-02 (Draft) INTERIOR AIR OF ROAD VEHICLES - PART 7: ODOUR DETERMINATION IN INTERIOR AIR OF ROAD VEHICLES AND TEST CHAMBER AIR OF TRIM COMPONENTS BY OLFACTORY MEASUREMENTS (ISO 12219-7:2017)
DIN ISO 16000-30:2015-05 INDOOR AIR - PART 30: SENSORY TESTING OF INDOOR AIR (ISO 16000-30:2014)
DIN ISO 16000-16:2009-12 Indoor air - Part 16: Detection and enumeration of moulds - Sampling by filtration (ISO 16000-16:2008)
DIN ISO 16000-14:2012-03 Indoor air - Part 14: Determination of total (gas and particle-phase) polychlorinated dioxin-like biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/PCDFs) - Extraction, clean-up and analysis by high-resolution gas chromatography and mass spectrometry (ISO 16000-14:2009)

VDI 4252 Blatt 2:2004-06 Measurement of airborne microorganisms and viruses in ambient air - Active sampling of bioaerosols - Separation of airborne mould on gelatine/polycarbonate filters
DIN EN 1040:2006-03 Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1); German version EN 1040:2005
EN 13098:2000 Workplace atmosphere - Guidelines for measurement of airborne micro-organisms and endotoxin
EN 1040:2005 Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - Test method and requirements (phase 1)

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