Microbiological examination for dairy purposes. Methods of general application for enumeration of microorganisms Enumeration of bacteria by direct microscopic counts

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

29-09-1989

Publisher

British Standards Institution

Foreword
Methods
1. Scope
2. Definitions
3. Principle
4. Method A. Direct microscopic count technique
5. Method B. Direct epifluorescent filter technique
(DEFT)
6. Test report
Table
1. Microscope factors
2. Quantities of sample, trypsin and surfactant
solutions for test

Two methods: A for total counts in milk and B (direct epifluorescent filter technique) for total counts and counts of \'active bacteria\' in milk and other samples.

This Section of BS 4285 describes two methods of general application for the direct enumeration of bacteria:

method A: direct microscopic count technique for total counts in milk;
method B: direct epifluorescent filter technique (DEFT) for total counts or counts of bacteria in milk, certain milk products and samples such as rinses.

NOTE 1. In samples (including plant rinses) that have received some degree of heat treatment (equivalent to pasteurisation or greater), certain organisms, particularly Gram-positive cocci, will fluoresce orange (i.e. as active) but will be non-viable by plate count methods. Therefore great care is needed in interpreting results using such samples.

NOTE 2. The titles of the publications referred to in this standard are listed on page 5.

Committee	AW/9
DevelopmentNote	Supersedes 88/52307 DC and BS 4285-2.5(1984). (08/2005) Reviewed and confirmed by BSI, December 2011. (11/2011)
DocumentType	Standard
Pages	6
PublisherName	British Standards Institution
Status	Current
Supersedes	BS 4285-2-2.5:1984

BS 4285-1.2:1984	Microbiological examination for dairy purposes. Guide to general procedures Diluents, media and apparatus and their preparation and sterilization
BS 4285-1.1(1984) : LATEST	MICROBIOLOGICAL EXAMINATION FOR DAIRY PURPOSES - GUIDE TO GENERAL PROCEDURES - SAMPLING AND PREPARATION OF SAMPLE
BS 4285-0:1984	Microbiological examination for dairy purposes General introduction
BS 4285-1.3:1984	Microbiological examination for dairy purposes. Guide to general procedures Procedures for obtaining incubation conditions

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BS 4285-2.5:1989

Microbiological examination for dairy purposes. Methods of general application for enumeration of microorganisms Enumeration of bacteria by direct microscopic counts

Table of Contents

Abstract

Scope

General Product Information

Standards Referencing This Book

Category

Subcategory