Name: CLSI I/LA26 A2 : 2ED 2013
Brand: CLSI
Price: 452.06 AUD

PERFORMANCE OF SINGLE CELL IMMUNE RESPONSE ASSAYS

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

27-11-2013

Publisher

Clinical Laboratory Standards Institute

Abstract
Committee Membership
Foreword
1 Scope
2 Standard Precautions
3 Terminology
4 Specimen Collection
5 Specimen Transport and Handling
6 Sample Preparation
7 Laboratory Procedure for the Assessment of
   Antigen-Specific Cellular Immune Responses Using
   Intracellular Cytokine Staining
8 Laboratory Procedure for the Assessment of
   Antigen-Specific Cellular Immune Responses Using
   Major Histocompatibility Complex Multimers
9 Laboratory Procedure for the Assessment of
   Antigen-Specific Cellular Immune Responses Using
   Enzyme-Linked Immunospot Technology
10 Laboratory Procedure for the Assessment of
   Antigen-Specific Cellular Immune Responses Using
   the Tracking Dye Carboxyfluorescein Succinimidyl Ester
11 Quality Assurance and Test Validation
12 Data Reporting
References
Appendix A - Cryopreservation of Viable Cells
Appendix B - Statistics of Rare-Event Analyses
Appendix C - Troubleshooting Table for Antigen-Specific
             Intracellular Cytokine Staining Assay
Appendix D - Troubleshooting Table for Major Histocompatibility
             Complex Multimer Assay
Appendix E - Troubleshooting Table for Enzyme-Linked
             Immunospot Assays
Appendix F - Troubleshooting Table for Carboxyfluorescein
             Succinimidyl Ester
The Quality Management System Approach
Related CLSI Reference Materials

This document provides guidance for the performance of single cell immune response assays within the clinical context of infectious diseases (especially HIV), cancer, transplantation, autoimmune disease, and allergies.

DevelopmentNote	Supersedes CLSI I/LA26 A. (12/2013)
DocumentType	Miscellaneous Product
ISBN	1-56238-893-2
Pages	106
PublisherName	Clinical Laboratory Standards Institute
Status	Current
Supersedes	CLSI I/LA26 A : 1ED 2004

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