EN 1104:2005
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
Paper and board intended to come into contact with foodstuffs - Determination of the transfer of antimicrobial constituents
22-02-2022
24-08-2005
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Apparatus
5.1 Punch iron
5.2 Pressing device
5.3 Zone reading device
5.4 Ordinary microbiological laboratory apparatus
6 Reagents
6.1 Water
6.2 Non ionic wetting agent,
6.3 Nutrient medium for Bacillus subtilis
6.4 Sabouraud modified mould nutrient medium for Aspergillus niger
6.5 Nutrient medium for inhibition test with Bacillus subtilis
6.6 Salt peptone solution
6.7 Test micro-organisms
6.7.1 General
6.7.2 Preparation of inoculating spore suspension of
Bacillus subtilis
6.7.3 Preparation of inoculating spore suspension of
Aspergillus niger
6.7.4 Concentrations of spores for inhibition test
6.8 Positive controls
6.8.1 Penicillin, G 0,03 units
6.8.2 Isothiazolinon-solution
7 Sampling and preparation of test pieces
8 Procedure
8.1 Sterilisation
8.2 Preparation of plates
8.2.1 General
8.2.2 Bacillus subtilis
8.2.3 Aspergillus niger
8.3 Incubation
9 Evaluation
10 Test report
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