Name: EN 15785:2009
Brand: CEN

Animal feeding stuffs - Isolation and enumeration of Bifidobacterium spp.

Published date

02-09-2009

Publisher

Comite Europeen de Normalisation

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Diluent, media and phenotypic characterisation
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
10 Expression of results
11 Precision
12 Test report
Annex A (informative) - Notes on procedures
Annex B (informative) - Results of the interlaboratory study
Bibliography

This European Standard defines general rules for the enumeration of probiotic bifidobacteria in feed samples (additives, premixtures and feeding stuffs) that contain bifidobacteria as a single bacterial component or in a mixture with other microorganisms. This standard is not applicable for mineral feeds which are defined as complementary feeding stuffs composed mainly of minerals and containing at least 40% crude ash (Council Directive 79/373/EEC) [3].There are different categories of feed samples:a)Additives containing about 1010 colony forming units (CFU)/gb)Premixtures containing about 108 CFU/gc)Feeds, meal or pellets, which contain about 106 CFU/g and include complete feeding stuffs, and milk replacers.The detection limit is as defined in ISO 7218.

Committee	CEN/TC 327
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
NEN EN 15785 : 2009	Identical
I.S. EN 15785:2009	Identical
SN EN 15785 : 2010	Identical
PN EN 15785 : 2009	Identical
BS EN 15785:2009	Identical
NF EN 15785 : 2009	Identical
UNI EN 15785 : 2009	Identical
DIN EN 15785 E : 2009	Identical
NS EN 15785 : 2009	Identical
DIN EN 15785:2009-12	Identical
UNE-EN 15785:2010	Identical
NBN EN 15785 : 2009	Identical

ISO 6887-1:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
EN ISO 6887-1:2017	Microbiology of the food chain - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 1: General rules for the preparation of the initial suspension and decimal dilutions (ISO 6887-1:2017)
ISO 6497:2002	Animal feeding stuffs Sampling
ISO 6498:2012	Animal feeding stuffs — Guidelines for sample preparation
EN ISO 7218 : 2007 COR 2014	MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS (ISO 7218:2007/AMD 1:2013, CORRECTED VERSION 2014-04-15)