Name: EN ISO 12966-4:2015
Brand: CEN

Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015)

Published date

10-06-2015

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Apparatus
6 Sampling
7 Preparation of test sample
8 Preparation of methyl esters
   from fats, oils, and fatty acids
9 Procedure
10 Calculations
11 Precision
12 Test report
Annex A (informative) - Theoretical flame ionization
        detector correction factor (TCF) for fatty
        acid methyl esters (FAMEs)
Annex B (informative) - Examples of chromatograms
Annex C (informative) - Comparison of FAME
        composition with two different GC columns
Annex D (informative) - Results of an interlaboratory trial
Bibliography

ISO 12966-4:2015 specifies a method for the determination of fatty acid methyl esters (FAMEs) derived by transesterification or esterification from fats, oils, and fatty acids by capillary gas chromatography (GLC). Fatty acid methyl esters from C8 to C24 can be separated using this part of ISO 12966 including saturated fatty acid methyl esters, cis- and trans-monounsaturated fatty acid methyl esters, and cis- and trans-polyunsaturated fatty acid methyl esters.The method is applicable to crude, refined, partially hydrogenated, or fully hydrogenated fats, oils, and fatty acids derived from animal and vegetable sources.This method is not suitable for the analysis of dairy, ruminant fats and oils, or products supplemented with conjugated linoleic acid (CLA). Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 12966.ISO 12966-4:2015 is not applicable to di-, tri-, polymerized and oxidized fatty acids, and fats and oils.

Committee	CEN/TC 307
DevelopmentNote	Supersedes EN ISO 5508 and EN ISO 15304. (06/2015)
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current
Supersedes	EN ISO 15304:2002/AC:2004 EN ISO 5508 : 1995

Standards	Relationship
NS EN ISO 12966-4 : 2015	Identical
I.S. EN ISO 12966-4:2015	Identical
PN EN ISO 12966-4 : 2015	Identical
SN EN ISO 12966-4:2015	Identical
UNI EN ISO 12966-4 : 2015	Identical
SS-EN ISO 12966-4:2015	Identical
UNE-EN ISO 12966-4:2015	Identical
BS EN ISO 12966-4:2015	Identical
DIN EN ISO 12966-4:2015-11	Identical
DIN EN ISO 12966-4 E : 2015	Identical
ISO 12966-4:2015	Identical
NF EN ISO 12966-4 : 2015	Identical
NBN EN ISO 12966-4 : 2015	Identical
NEN EN ISO 12966-4 : 2015	Identical

CFR 21(PTS100-169) : 0	FOOD AND DRUGS - FOOD AND DRUG ADMINISTRATION, CHAPTER 1 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PARTS 100-169
ISO 12966-2:2017	Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids
ISO 12966-3:2016	Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 3: Preparation of methyl esters using trimethylsulfonium hydroxide (TMSH)
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
ISO 661:2003	Animal and vegetable fats and oils Preparation of test sample