NEN EN ISO 21871 : 2006
Current
The latest, up-to-date edition.
MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - HORIZONTAL METHOD FOR THE ENUMERATION OF LOW NUMBERS OF PRESUMPTIVE BACILLUS CEREUS - MOST PROBABLE NUMBER TECHNIQUE AND DETECTION METHOD
12-01-2013
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
4.1 Enumeration method
4.2 Detection method
5 Diluent, culture media and reagents
5.1 General
5.2 Diluent
5.3 Liquid selective enrichment medium:
Tryptone soya polymyxin broth (TSPB)
5.4 Solid selective medium: Polymyxin
pyruvate egg yolk mannitol bromothymol
blue agar (PEMBA)
5.5 Solid selective medium: Mannitol egg
yolk polymyxin agar (MYP)
5.6 Staining solutions for microscopic
identification
5.7 Sheep blood agar
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Enumeration method
9.2 Detection method
10 Calculation and expression of results
10.1 Enumeration method for the determination
of the most probable number (MPN)
10.2 Detection method
11 Test report
Annex A (normative) Diagram of enumeration procedure
Bibliography
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