AS 5013.9-2009

This Standard sets out procedures for the examination of foods for coliforms and Escherichia coli by the triplicate tube method. This is a qualitative test and is suitable for determining the presence or absence of coliforms and E. coli in a stated quantity of material under test.A flow diagram of the procedures is shown in Appendix A.The method describes a general method and modifications may be required according to the material under test. Such modifications, if required, are prescribed in the method dealing with particular products.Where the test is to estimate the level of coliforms only as an index of potential spoilage of the product and of hygiene, an incubation temperature of 30°C is specified. However, where the test is to be extended to include the estimation or detection of E. coli, incubation at 37°C is specified. Cultures which have been incubated initially at 30°C cannot be used for further tests for E. coli.Methods for the examination of rinse fluids and filterable liquid products for coliforms and E. coli by membrane filtration are given in AS 5013.Quantitative coliform and E. coli methods are given in AS 5013.3 and AS 5013.15 respectively.

First published in part as AS 1095.3.1-1973.AS 1766.2 first published 1976.Revised and redesignated in part as AS 1766.2.3-1987.Second edition AS 1095.3.1-1987.AS 1766.2.3-1987 and AS 1095.3.1-1987 revised, amalgamated and designated AS 1766.2.3-1992.Revised and redesignated as AS 5013.9-2009. Originated in part as part of AS 1095.3.1 1973 and AS 1766.2 1976. Previous edition part of AS 1766.2.3 1992. Revised and redesignated as AS 5013.9 2009.