1 This document, along with ISO16000-38, specifies the measurement method for determining the mass concentration of primary, secondary and tertiary aliphatic and aromatic amines in indoor air using accumulated sampling and high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS-MS) or high-resolution mass spectrometry (HRMS). The analytical procedure is covered by this document. The sampling procedure and the manufacturing of the samplers are covered by ISO16000-38.This document describes specifications for the chromatography and the mass spectroscopy for the amines. Measurement results are expressed inµg/m3.Although primarily intended for the measurement of amines listed in TablesA.1 and A.2, it can also be used for the measurement of other amines in indoor air. This document gives instructions and describes procedures for the inclusion of other amines.The range of application of this document concerning the concentrations of amines in indoor air depends on the linear range of the calibration line and hence on the gas sample volume (here: from 5l up to 100l), the eluate volume (from 1ml up to 5ml), the injection volume (from 1µl up to 10µl) and the sensitivity of the analytical equipment (e.g. linear range from 2 pg up to 2 ng amine). The range of application can be expected to be from approximately 0,002µg/m3 (100l sample) up to 2000µg/m3 (5l sample) for a common analytical equipment (e.g. Waters „TQD“) for the majority of the amines listed in TablesA.1 and A.2. The analysis of derivatives of ethanolamine is usually about 10 times more sensitive and the analysis of short-chained aliphatic amines is usually about 10 times less sensitive than the analysis of an average amine.The performance data of the analytical method is given in Annex B, particularly in Tables B.1 and B.2.This document can be used also for the determination of amines in water if the detection limit is sufficient.This document does not cover the determination of isocyanates in indoor air (nor in water samples) as corresponding amines (covered by ISO17734-1 and ISO17734-2).