Name: EN 16160:2012
Brand: CEN

Animal feeding stuffs - Determination of Hydrocyanic acid by HPLC

Published date

14-03-2012

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Sampling
7 Preparation of test sample
8 Procedure
9 Calculation of results
10 Precision
11 Test report
Annex A (informative) - Results of the
        collaborative study
Annex B (informative) - Results of the
        collaborative study without recovery
        correction
Annex C (informative) - Alternative procedure
        for enzymatic breakdown (8.3.3)
        and steam distillation (8.3.4)
Bibliography

This European Standard is applicable to the quantitative analysis of (bound and free) hydrocyanic acid (HCN) in feed materials of plant origin and compound feed by High Performance Liquid Chromatography (HPLC).The method is validated from 10 mg HCN/kg to 350 mg HCN/kg. When the method is used outside this range it should be validated at least within the laboratory. A limit of quantification of 2 mg HCN/kg should normally be obtained.

Committee	CEN/TC 327
DevelopmentNote	Supersedes PREN 16160. (03/2012)
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
PN EN 16160 : 2012	Identical
NS EN 16160 : 2012	Identical
NEN EN 16160 : 2012	Identical
NF EN 16160 : 2012	Identical
UNE-EN 16160:2012	Identical
DIN EN 16160:2012-05	Identical
NBN EN 16160 : 2012	Identical
UNI EN 16160 : 2012	Identical
BS EN 16160:2012	Identical
SN EN 16160 : 2012	Identical
I.S. EN 16160:2012	Identical

ISO 6497:2002	Animal feeding stuffs Sampling
PREN ISO 6498 : DRAFT 2009	ANIMAL FEEDING STUFFS - GUIDELINES FOR SAMPLE PREPARATION
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
EN ISO 6497 : 2005	ANIMAL FEEDING STUFFS - SAMPLING