Name: EN ISO 16050:2011
Brand: CEN

Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method (ISO 16050:2003)

Published date

13-07-2011

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation of results
8 Precision
9 Test report
Annex A (informative) - Results of
interlaboratory test
Bibliography

ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B1, and for the sum of aflatoxins B1, B2, G1 and G2, is 8 micrograms per kilogram.The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.

Committee	CEN/TC 275
DevelopmentNote	Supersedes EN 12955. (07/2011)
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current
Supersedes	EN 12955 : 1999

Standards	Relationship
ISO 16050:2003	Identical
NF EN ISO 16050 : 2011	Identical
NS EN ISO 16050 : 2011	Identical
DIN EN ISO 16050:2011-09	Identical
NEN EN ISO 16050 : 2011	Identical
NBN EN ISO 16050 : 2011	Identical
PN EN ISO 16050 : 2011	Identical
UNI EN ISO 16050 : 2011	Identical
SS-EN ISO 16050 : 2011	Identical
UNE-EN ISO 16050:2011	Identical
I.S. EN ISO 16050:2011	Identical
BS EN ISO 16050:2011	Identical
SN EN ISO 16050:2011	Identical

ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 31-0:1992	Quantities and units Part 0: General principles
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 5725:1986	Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN 14123:2007	Foodstuffs - Determination of aflatoxin B1 and the sum of aflatoxin B1, B2, G1 and G2 in hazelnuts, peanuts, pistachios, figs, and paprika powder - High performance liquid chromatographic method with post-column derivatisation and immunoaffinity column cleanup