CLSI VET04 A : 1ED 2006
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
METHODS FOR BROTH DILUTION SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
09-25-2014
01-12-2013
Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Antimicrobial Agents
4.1 Source
4.2 Weighing Antimicrobial Powders
4.3 Preparing Stock Solutions
4.4 Number of Concentrations Tested
5 Selection of Antimicrobial Agents for Routine Testing
and Reporting
5.1 Routine Reports
5.2 Antimicrobial Classes
5.3 Suggested Guidelines for Use and Selective Testing
and Reporting
6 Indications for Performing Susceptibility Testing
7 Inoculum Preparation for Dilution Tests
7.1 Turbidity Standard for Inoculum Preparation
7.2 Direct Colony Suspension Method
8 Broth Dilution Procedures (Macrodilution and Microdilution)
8.1 Cation-Adjusted Mueller-Hinton Broth (CAMHB) Medium
8.2 Preparing and Storing Diluted Antimicrobial Agents
8.3 Broth Dilution Testing
9 Fastidious and Problem Organisms
9.1 Vibrionaceae and Photobacteriaceae (Obligate Halophilic
Strains) (Group 2)
9.2 Gliding Bacteria (Group 3)
9.3 Streptococci (Group 4)
9.4 Other Fastidious Organisms (Group 5)
10 Reporting of MIC Results
11 Quality Control Procedures
11.1 Purpose
11.2 Quality Control Responsibilities
11.3 Reference Strains for Quality Control
11.4 Storing and Testing Quality Control Strains
11.5 Control of Media and Materials
11.6 Broth Dilution Quality Control Ranges
11.7 Frequency of Quality Control Testing
11.8 Corrective Action
11.9 Other Control Procedures
12 Limitations of Broth Dilution Test Methods
12.1 Application to Various Organism Groups
References
Appendix A - Antimicrobial Agents Used in Global Aquaculture
and Status of Quality Control for Broth Dilution
Susceptibility Testing
Appendix B - Aerobic Dilution Daily Quality Control Testing
Protocol
Appendix C - Aerobic Dilution Weekly Quality Control Testing
Protocol
Summary of Comments and Subcommittee Responses
The Quality System Approach
Related CLSI/NCCLS Publications
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