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CLSI VET03 A : 1ED 2006

CLSI VET03 A : 1ED 2006

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

METHODS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS

Superseded date

03-30-2023

Published date

08-25-2018

Publisher

Clinical Laboratory Standards Institute

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Indications for Performing Susceptibility Testing
5 Selection of Antimicrobial Agents for Routine Testing
  and Reporting
  5.1 Routine Reports
  5.2 Antimicrobial Classes
  5.3 Suggested Guidelines for Use and Selective Testing
      and Reporting
6 Disk Diffusion Assay Protocol
  6.1 Media Preparation
  6.2 Inoculum
  6.3 Diffusion Disks
  6.4 Incubation
  6.5 Reading Plates
  6.6 Rejection Criteria
7 Fastidious and Problem Organisms
  7.1 Vibrionaceae and Photobacteriaceae (Obligate Halophilic
      Strains) (Group 2)
  7.2 Gliding Bacteria (Group 3)
  7.3 Streptococci (Group 4)
  7.4 Other Fastidious Organisms (Group 5)
8 Quality Control Procedures
  8.1 Purpose
  8.2 Reference Strains for Quality Control
  8.3 Accepted Quality Control Ranges
  8.4 Expansion of Control Strain Set
  8.5 Storing and Testing Quality Control Strains
  8.6 Control of Media and
  8.7 Frequency of Quality Control Testing
  8.8 Corrective Action
9 Quality Assurance Measures
References
Appendix A - Antimicrobial Agents Used in Global Aquaculture
             and Status of Quality Control for Disk Diffusion
             Susceptibility Testing
Appendix B - Fuzzy Zones and Halo Formation
Appendix C - Disk Diffusion Daily Quality Control Testing
             Protocol
Appendix D - Disk Diffusion Weekly Quality Control Testing
             Protocol
Summary of Delegate/Consensus Comments and Working Group
Responses
The Quality System Approach
Related CLSI/NCCLS Publications

Provides the most up-to-date techniques for disk diffusion susceptibility testing of aquatic species isolates, and criteria for quality control testing.

This CLSI guideline provides veterinary and aquatic animal disease diagnostic laboratories with currently recommended antimicrobial disk diffusion susceptibility testing methods for bacteria isolated from aquatic animals–primarily Group 1 organisms–including criteria for quality control testing with two quality control strains. The document also provides appendixes and tables outlining recommended disk concentrations, antimicrobial agents used in global aquaculture, methods for preparing stock solutions and dilutions of antimicrobial agents, and a list of bacteria pathogenic to fish.Interpretive criteria are not addressed in this guideline. Such criteria must be established using pharmacokinetic and pharmacodynamic data, in vitro susceptibility testing data, and clinical efficacy data. Developing interpretive criteria was beyond the scope of this document. As more aquatic animal-specific information becomes available, this document, and CLSI document M49—Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated From Aquatic Animals, will be revised to incorporate those data.

DevelopmentNote
Supersedes NCCLS M42 P (07/2006) Formerly CLSI M42 A. (07/2013)
DocumentType
Miscellaneous Product
PublisherName
Clinical Laboratory Standards Institute
Status
Superseded
Supersedes

Standards Relationship
CLSI VET03 2ED : 2020 Identical

CLSI VET04 A2 : 2ED 2014 METHODS FOR BROTH DILUTION SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
CLSI VET04 A : 1ED 2006 METHODS FOR BROTH DILUTION SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
CLSI VET01 A4 : 4ED 2013 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK AND DILUTION SUSCEPTIBILITY TESTS FOR BACTERIA ISOLATED FROM ANIMALS
CLSI VET03/VET04 S2 : 2ED 2014 PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS
CLSI VET06 : 1ED 2017 METHODS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF INFREQUENTLY ISOLATED OR FASTIDIOUS BACTERIA ISOLATED FROM ANIMALS
CLSI VET05 R : 1ED 2011 GENERATION, PRESENTATION, AND APPLICATION OF ANTIMICROBIAL SUSCEPTIBILITY TEST DATA FOR BACTERIA OF ANIMAL ORIGIN
CLSI VET02 A3 : 3ED 2008 DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS FOR VETERINARY ANTIMICROBIAL AGENTS

CLSI M23 A2 : 2ED 2001 DEVELOPMENT OF IN VITRO SUSPECTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M6 A2 : 2ED 2006 PROTOCOLS FOR EVALUATING DEHYDRATED MUELLER-HINTON AGAR
CLSI M31 A2 : 2ED 2002 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK AND DILUTION SUSCEPTIBILITY TESTS FOR BACTERIA ISOLATED FROM ANIMALS
CLSI M7 A7 : 7ED 2006 METHODS FOR DILUTION ANTIMICROBIAL SUSCEPTIBILITY TESTS FOR BACTERIA THAT GROW AEROBICALLY
CLSI M2 A9 : 9ED 2006 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS
CLSI M37 A2 : 2ED 2002 DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS FOR VETERINARY ANTIMICROBIAL AGENTS

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