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CLSI M42 P : 1ED 2005

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

METHODS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTING OF BACTERIA ISOLATED FROM AQUATIC ANIMALS

Superseded date

06-20-2006

Published date

01-12-2013

Abstract
Committee Membership
Foreword
1 Scope
2 Introduction
3 Definitions
4 Indications for Performing Susceptibility Testing
5 Selection of Antimicrobial Agents for Routine Testing and
  Reporting
  5.1 Routine Reports
  5.2 Antimicrobial Classes
  5.3 Suggested Guidelines for Use and Selective Testing and
      Reporting
6 Disk Diffusion Assay Protocol
  6.1 Media Preparation
  6.2 Inoculum
  6.3 Diffusion Disks
  6.4 Incubation
  6.5 Reading Plates
  6.6 Rejection Criteria
7 Fastidious and Problem Organisms
  7.1 Obligate Halophilic Strains (Group 2)
  7.2 Gliding Bacteria (Group 3)
  7.3 Streptococci (Group 4)
  7.4 Other Fastidious Organisms (Group 5)
8 Quality Control Procedures
  8.1 Purpose
  8.2 Reference Strains for Quality Control
  8.3 Accepted Quality Controls Ranges
  8.4 Expansion of Control Strain Set
  8.5 Storing and Testing Quality Control Strains
  8.6 Control of Media and Disks
  8.7 Frequency of Quality Control Testing
  8.8 Corrective Action
9 Quality Assurance Measures
References
Appendix A. Antimicrobial Agents Used in Global Aquaculture
            and Status of Quality Control for Disk Diffusion
            Susceptibility Testing
Appendix B. Fuzzy Zones and Halo Formation
Appendix C. Disk Diffusion Daily Quality Control Testing
            Protocol
Appendix D. Disk Diffusion Weekly Quality Control Testing
            Protocol
Tables
The Quality System Approach
Related CLSI/NCCLS Publications

Describes the standard agar disk diffusion method and quality control criteria for testing Group 1 aquatic bacteria.

DevelopmentNote
Supersedes NCCLS M42 R. (09/2005)
DocumentType
Proposed Guideline
PublisherName
Clinical Laboratory Standards Institute
Status
Superseded
SupersededBy
Supersedes

CLSI M23 A2 : 2ED 2001 DEVELOPMENT OF IN VITRO SUSPECTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M31 A2 : 2ED 2002 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK AND DILUTION SUSCEPTIBILITY TESTS FOR BACTERIA ISOLATED FROM ANIMALS
CLSI M2 A8 : 8ED 2003 PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS
CLSI M37 A2 : 2ED 2002 DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS FOR VETERINARY ANTIMICROBIAL AGENTS

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