AS/NZS 1766.2.16.1:1998

1 - AS/NZS 1766.2.16.1:1998 FOOD MICROBIOLOGY - EXAMINATION FOR SPECIFIC ORGANISMS-FOOD AND ANIMAL FEEDING STUFFS-HORIZONTAL ....
1 - PREFACE
2 - CONTENTS
3 - 1 Scope
3 - 2 Normative references
3 - 3 Definitions
3 - 3.1 Listeria monocytogenes:
3 - 3.2 detection of Listeria monocytogenes:
4 - 4 Principle
4 - 4.1 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half Fraser broth
4 - 4.2 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4 - 4.3 Plating out and identification
4 - 4.4 Confirmation
4 - 5 Culture media and reagents
5 - 6 Apparatus and glassware
5 - 6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
5 - 6.2 Drying cabinet or incubator,
5 - 6.3 Incubators,
5 - 6.4 Water bath,
5 - 6.5 Loops,
5 - 6.6 pH-meter,
5 - 6.7 Test tubes or flasks,
5 - 6.8 Measuring cylinders,
5 - 6.9 Total-delivery graduated pipettes,
5 - 6.10 Petri dishes,
5 - 6.11 Jars,
5 - 6.12 Gas mixture
5 - 6.13 Equipment for the Henry illumination test (optional).
5 - 6.14 Microscope,
5 - 7 Sampling
5 - 8 Preparation of test sample
5 - 9 Procedure
5 - 9.1 Test portion and initial suspension
6 - 9.2 Primary enrichment
6 - 9.3 Secondary enrichment
6 - 9.4 Plating out and identification
6 - 9.5 Confirmation of Listeria spp.
6 - 9.5.1 Selection of colonies for confirmation
7 - 9.5.2 Catalase reaction
7 - 9.5.3 Gram staining
7 - 9.5.4 Motility test (if necessary)
7 - 9.6 Confirmation of L. monocytogenes
7 - 9.6.1 Haemolysis test
7 - 9.6.2 Carbohydrate utilization
7 - 9.6.3 CAMP test
8 - 9.7 Interpretation of morphological and physiological properties and of the biochemical reactions
8 - 9.8 Definitive confirmation
9 - 9.9 Control cultures
9 - 10 Expression of results
9 - 11 Test report
10 - Annex A - Diagram of procedure
11 - Annex B - Composition and preparation of culture media and reagents
11 - B.1 Selective primary enrichment medium: Halt Fraser broth
12 - B.2 Selective secondary enrichment medium: Fraser broth
13 - B.3 First selective plating-out medium: Oxford agar
13 - B.4 Second selective plating-out medium: PALCAM agar
14 - B.5 Solid culture medium: Tryptone soya yeast extract agar (TSYEA)
15 - B.6 Liquid culture medium: Tryptone soya yeast extract broth (TSYEB)
15 - B.7 Sheep blood agar
15 - B.8 Carbohydrate utilization broth (rhamnose and xylose)
16 - B.9 Motility agar
16 - B.10 CAMP (Christie, Atkins, Munch-Petersen) medium and test strains
16 - B.11 Hydrogen peroxide solution
17 - B.12 Phosphate-buffered saline (PBS)
18 - Annex C - HENRY ILLUMINATION TEST
19 - ANNEX ZZ - ADDITIONAL REQUIREMENTS TO ISO 11290-1:1996 FOR AUSTRALIAN/NEW ZEALAND CONDITIONS
19 - ZZ1 SCOPE
19 - ZZ2 ADDITIONAL REQUIREMENTS