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BS EN ISO 21871:2006

BS EN ISO 21871:2006

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Microbiology of food and animal feeding stuffs. Horizontal method for the determination of low numbers of presumptive Bacillus cereus. Most probable number technique and detection method

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

02-01-2006

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 Enumeration method
   4.2 Detection method
5 Diluent, culture media and reagents
   5.1 General
   5.2 Diluent
   5.3 Liquid selective enrichment medium:
        Tryptone soya polymyxin broth (TSPB)
   5.4 Solid selective medium: Polymyxin
        pyruvate egg yolk mannitol bromothymol
        blue agar (PEMBA)
   5.5 Solid selective medium: Mannitol egg
        yolk polymyxin agar (MYP)
   5.6 Staining solutions for microscopic
        identification
   5.7 Sheep blood agar
6 Apparatus and glassware
7 Sampling
8 Preparation of test sample
9 Procedure
   9.1 Enumeration method
   9.2 Detection method
10 Calculation and expression of results
   10.1 Enumeration method for the determination
        of the most probable number (MPN)
   10.2 Detection method
11 Test report
Annex A (normative) Diagram of enumeration procedure
Bibliography

Describes a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique.

This International Standard specifies a horizontal method for the detection or the enumeration of low numbers of viable presumptive Bacillus cereus by means of the most probable number technique. This International Standard is applicable to

  • products intended for human consumption and the feeding of animals, and

  • environmental samples in the area of food production and food handling.

Committee
AW/9
DevelopmentNote
Supersedes 04/30110927 DC. (02/2006)
DocumentType
Standard
Pages
26
PublisherName
British Standards Institution
Status
Current
Supersedes

ISO/TS 11133-1:2009 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO/TS 11133-2:2003 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 2: Practical guidelines on performance testing of culture media
ISO 8261:2001 Milk and milk products General guidance for the preparation of test samples, initial suspensions and decimal dilutions for microbiological examination

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Hardcopy - English
PDF - English
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