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ASTM F 3106 : 2014

ASTM F 3106 : 2014

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Standard Guide for in vitro Osteoblast Differentiation Assays

Available format(s)

Hardcopy , PDF

Superseded date

04-29-2022

Language(s)

English

Published date

02-11-2015

Publisher

American Society for Testing and Materials

CONTAINED IN VOL. 13.02, 2015 Gives guidance on how to conduct in vitro osteoblast differentiation assays with progenitor stem cells including mesenchymal stromal cells.

1.1This document provides guidance on how to conduct in vitro osteoblast differentiation assays with progenitor stem cells including mesenchymal stromal cells.

1.2This document describes the roles of various osteogenic supplements that are added to the cell culture medium of an osteoblast differentiation assay to encourage and support the differentiation of progenitor cells into matrix-producing osteoblasts.

1.3This document provides recommendations for the concentrations of osteogenic supplements that may prevent the precipitation of artifactual mineral deposits that are not directly produced by osteoblasts, nor correlated with osteoblastic gene expression of the cells.

1.4This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Committee
F 04
DocumentType
Guide
Pages
5
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

ASTM F 3510 : 2021 Standard Guide for Characterizing Fiber-Based Constructs for Tissue-Engineered Medical Products

ASTM F 2312 : 2011 : REDLINE Standard Terminology Relating to Tissue Engineered Medical Products
ASTM F 2312 : 2004 Standard Terminology Relating to Tissue Engineered Medical Products
ASTM F 2312 : 2011 Standard Terminology Relating to Tissue Engineered Medical Products
ASTM F 2312 : 2011 : R2020 Standard Terminology Relating to Tissue Engineered Medical Products
ASTM F 2312 : 2010 Standard Terminology Relating to Tissue Engineered Medical Products
ASTM F 2997 : 2021 Standard Practice for Quantification of Calcium Deposits in Osteogenic Culture of Progenitor Cells Using Fluorescent Image Analysis
ASTM F 2997 : 2013 Standard Practice for Quantification of Calcium Deposits in Osteogenic Culture of Progenitor Cells Using Fluorescent Image Analysis
ASTM F 2312 : 2003 Standard Terminology Relating to Tissue Engineered Medical Products

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Hardcopy - English
PDF - English
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