Methods of analysis of fats and fatty oils. Other methods Detection and identification of antioxidants

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Hardcopy , PDF

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English

Published date

04-29-1983

Publisher

British Standards Institution

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Describes thin-layer chromatography for nordihydroguairetic, propylgallate, octylgallate, dodecylgallate, tertiobutylhydroquinone, butylhydroxyanisole, α-tocopherol and butylhydroxytoluene.

This International Standard specifies a thin-layer chromatographic method for the detection and identification of the following antioxidants in animal and vegetable fats and oils:

nordihydroguairetic acid (NDGA)
propylgallate (PG)
octylgallate (OG)
dodecylgallate (DG)
tertiobutylhydroquinone (TBHQ)
butylhydroxyanisole (tert-butyl-4-methoxyphenol) (BHA)
α-tocopherol
butylhydroxytoluene (2,6-di-tert-butyl-4-methylphenol) (BHT)

The antioxidants are listed in the order of their R_f values.

The detection of α-tocopherol is sometimes difficult as the spots of this antioxidant are of a generally diffuse aspect.

The method has limits of detection of 20 mg/kg (ppm) for BHT, and 10 mg/kg (ppm) for the other antioxidants. It should be noted that BHT is not completely isolated by this method.

Committee	AW/307
DevelopmentNote	Also numbered as ISO 5558 Supersedes 81/51638 DC (09/2005)
DocumentType	Standard
Pages	8
PublisherName	British Standards Institution
Status	Current

BS 684-2.44:1984	Methods of analysis of fats and fatty oils. Other methods Determination of gallates
BS 684-2.36:1983	Methods of analysis of fats and fatty oils. Other methods Determination of butylhydroxyanisole (BHA) and butylhydroxytoluene (BHT)

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BS 684-2.33:1983

Methods of analysis of fats and fatty oils. Other methods Detection and identification of antioxidants

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