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BS EN 14569:2004

BS EN 14569:2004

Current

Current

The latest, up-to-date edition.

Foodstuffs. Microbiological screening for irradiated food using LAL/GNB procedures

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

10-15-2004

Publisher

British Standards Institution

Foreword
1 Scope
2 Normative references
3 Principle
4 Procedures
5 Evaluation
6 Limitations
7 Validation
8 Test report
Annex A (normative) Enumeration of total viable Gram negative
        bacteria (GNB)
Annex B (normative) Determination of endotoxin concentration
        using the Limulus amoebocyte lysate (LAL) test
Annex C (informative) Figures
Bibliography

Describes the microbiological screening method comprising two procedures, which are carried out in parallel.

This European Standard specifies a microbiological screening method comprising two procedures, which are carried out in parallel. It permits the identification of an unusual microbiological profile in poultry meat. The presence of a large excess population of dead micro-organisms can under certain circumstances be presumptive of irradiation treatment, which means, that the results of the procedure of the determination of endotoxin concentration in the test sample using the Limulus amoebocyte lysate (LAL) test and of the procedure of the enumeration of total Gram negative bacteria (GNB) in the test sample are not radiation specific. Therefore, it is recommended that a positive result be confirmed using a standardized reference method for the detection of irradiated food, e.g. EN 1784, EN 1785 or EN 1786 [1] to [3]. This screening method has been successfully tested by inter-laboratory trials [4], [5], [6] and the procedure is generally applicable to whole or parts of poultry, e.g. breast, legs, wings of fresh, chilled or frozen carcasses with or without skin. The method can also provide information about the microbiological quality of a product prior to irradiation.

Committee
AW/275
DevelopmentNote
Supersedes 02/710050 DC. (10/2004)
DocumentType
Standard
Pages
22
PublisherName
British Standards Institution
Status
Current
Supersedes

Standards Relationship
UNI EN 14569 : 2005 Identical
NEN EN 14569 : 2004 Identical
NS EN 14569 : 1ED 2005 Identical
UNE-EN 14569:2005 Identical
SN EN 14569 : 2005 Identical
NF EN 14569 : 2005 Identical
NBN EN 14569 : 2004 Identical
I.S. EN 14569:2004 Identical
EN 14569 : 2004 Identical
DIN EN 14569 E : 2005 Identical
DIN EN 14569:2005-01 Identical

ISO/TS 11133-1:2009 Microbiology of food and animal feeding stuffs Guidelines on preparation and production of culture media Part 1: General guidelines on quality assurance for the preparation of culture media in the laboratory
ISO 7218:2007 Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
EN 1785:2003 Foodstuffs - Detection of irradiated food containing fat - Gas chromatographic/mass spectrometric analysis of 2-alkylcyclobutanones
EN 1784:2003 Foodstuffs - Detection of irradiated food containing fat - Gas chromatographic analysis of hydrocarbons
ENV ISO 11133-1 : DRAFT 2000 MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GUIDELINES ON PREPARATION AND PRODUCTION OF CULTURE MEDIA - PART 1: GENERAL GUIDELINES ON QUALITY ASSURANCE FOR THE PREPARATION OF CULTURE MEDIA IN THE LABORATORY
EN 1786:1996 Foodstuffs - Detection of irradiated food containing bone - Method by ESR spectroscopy

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