Customer Support: +1 416-401-8730

Login to i2i Subscription Intertek.com
  • There are no items in your cart
Please enter a keyword to search
Advanced search
Home
BSI
BS EN ISO 22174:2005

BS EN ISO 22174:2005

Current

Current

The latest, up-to-date edition.

Microbiology of food and animal feeding stuffs. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. General requirements and definitions

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

03-24-2005

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
   4.1 General
   4.2 Preliminary microbial enrichment
   4.3 Nucleic acid preparation
   4.4 PCR amplification
   4.5 Detection and confirmation of PCR products
5 Test material
6 General laboratory requirements
   6.1 General
   6.2 Personnel
   6.3 Laboratory setup
   6.4 Waste management
7 Reagents
8 Apparatus and equipment
   8.1 General
   8.2 Special considerations
9 Procedure
   9.1 Sample preparation
   9.2 Amplification
   9.3 Control reaction
   9.4 Confirmation of PCR results
10 Evaluation
11 Test report
Bibliography

Describes the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). Applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR).

This International Standard gives the general requirements for the in vitro amplification of nucleic acid sequences (DNA or RNA). It is applicable to the testing of foodstuffs and isolates obtained from foodstuffs for food-borne pathogens using the polymerase chain reaction (PCR).

The minimum requirements laid down in this International Standard are intended to ensure that comparable and reproducible results are obtained in different laboratories.

This International Standard has been established for food-borne pathogens in or isolated from food and feed matrices, but is also applicable to other matrices (e.g. environmental samples) and for the detection of non-pathogenic microorganisms.

Committee
AW/9
DevelopmentNote
Supersedes 02/706195 DC. (04/2005)
DocumentType
Standard
Pages
22
PublisherName
British Standards Institution
Status
Current
Supersedes

ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
ISO 5725-1:1994 Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 20837:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 20838:2006 Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 3534-1:2006 Statistics — Vocabulary and symbols — Part 1: General statistical terms and terms used in probability

View more information
US$160.51
Excluding Tax where applicable
USD
CAD
CHF
CNY
DKK
EUR
GBP
HKD
IDR
INR
JPY
KRW
MXN
NOK
NZD
SEK
SGD
USD
ZAR
Hardcopy - English
PDF - English
01
02
03
04
05
06
07
08
09
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50

Access your standards online with a subscription

Features

  • Simple online access to standards, technical information and regulations.

  • Critical updates of standards and customisable alerts and notifications.

  • Multi-user online standards collection: secure, flexible and cost effective.

Learn more on subscription solutions

or

Get in touch with us