Name: CEN ISO/TS 17919:2013
Brand: CEN

Microbiology of the food chain - Polymerase chain reaction (PCR) for the detection of food-borne pathogens - Detection of botulinum type A, B, E and F neurotoxin-producing clostridia (ISO/TS 17919:2013)

Published date

11-06-2013

Publisher

Comite Europeen de Normalisation

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Symbols and abbreviated terms
5 Principle
6 Reagents
7 Apparatus and equipment
8 Sampling
9 Procedure
Annex A (normative) - Flow-diagram of the procedure
Annex B (informative) - Multiplex PCR assays for detection
        of genes encoding botulinum neurotoxin types A, B, E,
        and F using agarose gel electrophoresis
Annex C (informative) - Assays for detection of genes
        encoding botulinum neurotoxin types A, B, E, and
        F using real-time PCR
Annex D (informative) - Preparation of C. botulinum spores
Bibliography

ISO/TS 17919:2013 specifies a horizontal method for the molecular detection of clostridia carrying botulinum neurotoxin A, B, E, and F genes by a PCR method. This method detects the genes and not the toxins, therefore a positive result does not necessarily mean the presence of these toxins in the sample investigated. ISO/TS 17919:2013 is applicable to products for human consumption, animal feed, and environmental samples.The PCR assays for detection of genetic sequences encoding specific toxin types are described in annexes.

Committee	CEN/TC 463
DocumentType	Technical Specification
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
DIN CEN ISO/TS 17919;DIN SPEC 10793:2014-03	Identical
DIN CEN ISO/TS 17919;DIN SPEC 10793:2014-03	Identical
UNI CEN ISO/TS 17919 : 2013	Identical
XP CEN ISO/TS 17919 : 2013 XP	Identical
S.R. CEN ISO/TS 17919:2013	Identical
NEN NPR CEN ISO/TS 17919 : 2013	Identical
PD CEN ISO/TS 17919:2013	Identical
UNE-CEN ISO/TS 17919:2013	Identical
ISO/TS 17919:2013	Identical

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ISO 6887-1:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 6887-6:2013	Microbiology of food and animal feed — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 6: Specific rules for the preparation of samples taken at the primary production stage
ISO 20837:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 22118:2011	Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics
ISO 6887-5:2010	Microbiology of food and animal feeding stuffs Preparation of test samples, initial suspension and decimal dilutions for microbiological examination Part 5: Specific rules for the preparation of milk and milk products
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 6887-4:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 4: Specific rules for the preparation of miscellaneous products
ISO 6887-3:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products
ISO 6887-2:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 2: Specific rules for the preparation of meat and meat products
ISO 22119:2011	Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions